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Why we use caffeine for HPLC calibration in Detector wave
ength accuracy test
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caffeiene having different wavelents it having 2 maximas
and 1 minima.
1st maxima is 205nm
2nd maxima is 273nm
minima is 245nm
and it is primary reference standard and also suggested in
pharmacopiea.
| Is This Answer Correct ? | 379 Yes | 13 No |
Answer / satya prasad
it will detect at dual wave length and easily available in
market
| Is This Answer Correct ? | 253 Yes | 56 No |
Answer / atul ingle
Why we use it, Because of
# It gives a multiwavelength response like 205,245,273 nm
# It is easily available in market
# It is very stable, we can use a long time foranalysis
i.e. it is not degradable
# It is chip
| Is This Answer Correct ? | 173 Yes | 13 No |
Answer / devendrakumar
1. caffeine easily available in market.
2. In general we all use caffeine as a standard for
calibration of HPLC specially for (Calibration of Wave
Length Accuracy) since it will scan the whole of the UV
range i.e It gives a multiwavelength response like
first maxima at 205 nm
second maxima at 273 nm &
minima at 245nm
3. It is very stable, we can use a long time for analysis
i.e. it is not degradable
4. In this case we can use Erbium Perchlorate which will
scan the whole range(200 nm to 600 nm).It is stable and
little costly but not easily available in market.
d.devendrakumar
| Is This Answer Correct ? | 100 Yes | 5 No |
Answer / amblimath
# It detectable in multi wavelengths, 205,245,275
# Anhydrous Caffeine is primary standard.
# It contains nonrecemic CHON
# Easily available and can be stored ( no degradation)
| Is This Answer Correct ? | 47 Yes | 12 No |
Answer / sunil tiwari
# It detects on multiple wavelengths, 205,254,273.
# Anhydrous Caffeine is primary standard.
# Highly stable in solution form & can be used for multiple
days.
# Recomennded by many pharmacopoeia.
By Sunil Tiwari Lupin Limited Bhopal
| Is This Answer Correct ? | 47 Yes | 18 No |
Answer / ravivardhan
IT WILL BE DETECT DIFERENT WAVE LENGTHS EASILY AVAILBLE IN
MARKET
| Is This Answer Correct ? | 49 Yes | 25 No |
Answer / kamesh
It is a primary standard and it will detect at dual wave
length
| Is This Answer Correct ? | 33 Yes | 10 No |
Answer / kishanpal singh
caffeiene having different wavelents it having 2 maximas
and 1 minima.
1st maxima is 205nm
2nd maxima is 273nm
minima is 245nm
and it is primary reference standard and also suggested in
pharmacopiea.and avialable easly to market
long time store this
| Is This Answer Correct ? | 24 Yes | 2 No |
Answer / latif ahmed
1.caffeine is available in anhydrous form.
2.Shows maxima at two wavelengths ie.205nm & 273nm
3.Cheap & easily avalable.
| Is This Answer Correct ? | 15 Yes | 2 No |
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Organic Chemistry 
