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Why we use caffeine for HPLC calibration in Detector wave
ength accuracy test
- 60 Answers
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Answer / anil ramnani
Caffeine is used because it gives maxima at 272 and 205 nm and minima at 245 nm Wavelength, and it's preparation is stable at room temperature, and it is soluble in water and easily available.
| Is This Answer Correct ? | 1 Yes | 0 No |
Answer / ch prasad drl
1 IT DETECT A MULTIWAVELENGTH 1.205 2. 273 3. 245.
2 AND IT IS PRIMARY REFERENCE STANDARD.
3 IT IS VERY STABLE
4 IT IS VERY EASIY AVAILABLE IN MARCKET
5 IT IS CHEAP.
| Is This Answer Correct ? | 1 Yes | 0 No |
Answer / kishanpal singh, fare labs pvt
1. caffeine easily available in market.
2. In general we all use caffeine as a standard for
calibration of HPLC specially for (Calibration of Wave
Length Accuracy) since it will scan the whole of the UV
range i.e It gives a multiwavelength response like
first maxima at 205 nm
second maxima at 273 nm &
minima at 245nm
3. It is very stable, we can use a long time for analysis
i.e. it is not degradable
4. In this case we can use Erbium Perchlorate which will
scan the whole range(200 nm to 600 nm).It is stable and
little costly but not easily available in market.
KISHANPAL SINGH
| Is This Answer Correct ? | 1 Yes | 0 No |
Answer / suresh m.u.g. dr.reddy's
1.It gives a multiwavelength response at 205,245,273 nm
2.Suggested pharmacopoeial standard
3.Low cost and freely available in market.
| Is This Answer Correct ? | 1 Yes | 0 No |
Answer / prashant ghadi
1)Caffiene is primary standard
2)It is highly stable. also stable in solution form
3)It is polar compound. retention time is smaller
4)Easily available in the market, cheap
5)It has 2 maximas and 1 minima.
| Is This Answer Correct ? | 1 Yes | 0 No |
Answer / k.rajasekhara reddy
It is different wavelengths two maxima and one minima wavelengths 204, 272,245nm. this is no degradation due to environmental factors easily available and low cost in market
| Is This Answer Correct ? | 1 Yes | 0 No |
Answer / m . naga swamy.
It is the primary standard and recommended by the
pharmacopoeia. It is easily soluble in water. It shows
complete absorbance at multiwavelength
| Is This Answer Correct ? | 5 Yes | 5 No |
Answer / suryabhan datir
caffeiene having different wavelents it having 2 maximas
and 1 minima.
1st maxima is 205nm
2nd maxima is 273nm
minima is 245nm
and it is primary reference standard and also suggested in
pharmacopiea.It is easily soluble in water. It shows
complete absorbance at multiwavelength
| Is This Answer Correct ? | 3 Yes | 3 No |
Answer / abhijeet gavali
Caffeine is uv-visible Tracer thats why we are using
Caffeine for detector Calibration
| Is This Answer Correct ? | 0 Yes | 0 No |
Answer / vijay kadam
I think the answer no. 31 is absolutely correct
| Is This Answer Correct ? | 0 Yes | 0 No |
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