Why we use caffeine for HPLC calibration in Detector wave
ength accuracy test
- 60 Answers
- 191421 Views
- Arvind, Aurobindo, Cipla, Reddy Labs, Shasun Chemicals, I also Faced
- E-Mail Answers
Answers were Sorted based on User's Feedback
Answer / anil ramnani
Caffeine is used because it gives maxima at 272 and 205 nm and minima at 245 nm Wavelength, and it's preparation is stable at room temperature, and it is soluble in water and easily available.
| Is This Answer Correct ? | 1 Yes | 0 No |
Answer / ch prasad drl
1 IT DETECT A MULTIWAVELENGTH 1.205 2. 273 3. 245.
2 AND IT IS PRIMARY REFERENCE STANDARD.
3 IT IS VERY STABLE
4 IT IS VERY EASIY AVAILABLE IN MARCKET
5 IT IS CHEAP.
| Is This Answer Correct ? | 1 Yes | 0 No |
Answer / kishanpal singh, fare labs pvt
1. caffeine easily available in market.
2. In general we all use caffeine as a standard for
calibration of HPLC specially for (Calibration of Wave
Length Accuracy) since it will scan the whole of the UV
range i.e It gives a multiwavelength response like
first maxima at 205 nm
second maxima at 273 nm &
minima at 245nm
3. It is very stable, we can use a long time for analysis
i.e. it is not degradable
4. In this case we can use Erbium Perchlorate which will
scan the whole range(200 nm to 600 nm).It is stable and
little costly but not easily available in market.
KISHANPAL SINGH
| Is This Answer Correct ? | 1 Yes | 0 No |
Answer / suresh m.u.g. dr.reddy's
1.It gives a multiwavelength response at 205,245,273 nm
2.Suggested pharmacopoeial standard
3.Low cost and freely available in market.
| Is This Answer Correct ? | 1 Yes | 0 No |
Answer / prashant ghadi
1)Caffiene is primary standard
2)It is highly stable. also stable in solution form
3)It is polar compound. retention time is smaller
4)Easily available in the market, cheap
5)It has 2 maximas and 1 minima.
| Is This Answer Correct ? | 1 Yes | 0 No |
Answer / k.rajasekhara reddy
It is different wavelengths two maxima and one minima wavelengths 204, 272,245nm. this is no degradation due to environmental factors easily available and low cost in market
| Is This Answer Correct ? | 1 Yes | 0 No |
Answer / m . naga swamy.
It is the primary standard and recommended by the
pharmacopoeia. It is easily soluble in water. It shows
complete absorbance at multiwavelength
| Is This Answer Correct ? | 5 Yes | 5 No |
Answer / suryabhan datir
caffeiene having different wavelents it having 2 maximas
and 1 minima.
1st maxima is 205nm
2nd maxima is 273nm
minima is 245nm
and it is primary reference standard and also suggested in
pharmacopiea.It is easily soluble in water. It shows
complete absorbance at multiwavelength
| Is This Answer Correct ? | 3 Yes | 3 No |
Answer / abhijeet gavali
Caffeine is uv-visible Tracer thats why we are using
Caffeine for detector Calibration
| Is This Answer Correct ? | 0 Yes | 0 No |
Answer / vijay kadam
I think the answer no. 31 is absolutely correct
| Is This Answer Correct ? | 0 Yes | 0 No |
In the gas chromatography what is used carrier gas and make up gas?
what is hold time in Gc?
what is the difference between melting range & melting point? Which is more significant?
Why a1% value is used for some product ? What is the criteria for selection a1% value ?
WHAT IS THE PARAMETER FOR THE SELECTION OF MOBILE PHASE IN HPLC?
what r the reasons to get peak telling?
is a bonded water molecule in an organic material can be easlisy and accuractly determined by KF rather than LOD
How can we calculate offset value pH measurment
If we have a kc is1*10^8 and the .kp 2*10^5 of reaction A------->B C------->D what is the volume of C?
why we use polystyrene film for ftir calibration?
what is procedure of actual procedure for registration of drug?
why we use pyridine to determine moisture content of acetonel
Organic Chemistry 
