Why we use caffeine for HPLC calibration in Detector wave
ength accuracy test
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Answer / j vinay kumar
caffine observed at differnt wave lenth
and it s cheap and natural product
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It,is a primary standard.and very stable
it can detect multiple wave lenths. another standards also there
examples are benzophenone,uracini,harbium perchlorate extra
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Answer / narayanan
# Potasium dichromate in perchloric acid and Halmium oxide
in perchloric acid can be used for calibration.
# But, after calibration it takes long time to get neutral
range ( sinceacidic in nature).
# lot of washing procedure needs to be followed.
# caffeine is water soluble and easily removable.
| Is This Answer Correct ? | 1 Yes | 1 No |
Answer / sreehari kumar annamraju
caffeiene having different wavelents it having 2 maximas
and 1 minima.
1st maxima is 205nm
2nd maxima is 273nm
minima is 245nm
and it is primary reference standard and also suggested in
pharmacopiea.It is easily soluble in water. It shows
complete absorbance at multiwavelength,caffeine easily available in market.
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Answer / ram vishal verma
# It detectable at two maxima and one minima.
# Anhydrous Caffeine is primary standard.
# Caffeine is available in highly pure and stable in
solution form.
# Recomennded in Many pharmacopoeia.
By Ram Vishal Verma Otsuka Chemical India Pvt.Limited
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Answer / foestar fernandes. lupin lim
1) Its pharmacopia recomended
2)Highly stable in solution form
3)Its wavelengths are known(2 maximas at 205 and 273 also 1 minima at 245)
4)Cheap and readily available
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Answer / raja
The actual thing is, we need to get same absorbence at
differnt wavelength, it is possible only for caffiene
so that we are prefare for HPLC calibration.
and caffiene is available in market.
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Answer / balasaheb pawar
1)Caffiene gives minima at 205 nm and maxima 273 nm.
2) caffiene is diluted,elution easily and faster
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Answer / surekha
caffiene is available in pure state and it is a primary
standard .
| Is This Answer Correct ? | 0 Yes | 0 No |
caffine is primary standar recomanded in various
pharmacopia and it shows dual behaviour having different
wavelength 2 maxima 205nm,273nm and minima at 245nm.
SOM CHAUHAN R.K.LABORATORIES
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