Why we use caffeine for HPLC calibration in Detector wave
ength accuracy test
- 60 Answers
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Answer / pranav desai
1#It is primary reference stanard.
2#It is suggested in US,IcH Pharmacopiea.
3#It is 2 Mixima and 1 Minima
(205nm,273nm)(245nm)
4#it is eslyli soluble in solvent.
5# it is less carry over.
| Is This Answer Correct ? | 1 Yes | 2 No |
Answer / ravi kiran
it will detect at dual wave length and easily available in
market not degrade
| Is This Answer Correct ? | 0 Yes | 1 No |
Answer / satishchandra m.patel
2 maximas
and 1 minima
1st maxima is 205nm
2nd maxima is 273nm
minima is 245nm
also suggested in pharmacopiea
caffeine easily available in marketIt is easily soluble in
water
| Is This Answer Correct ? | 1 Yes | 3 No |
Answer / k.g.gupta dr.reddys
Most of the Pharmaceutical products are analysing in the
range of 200-300nm. Caffiene is having 2maxima and 1
minima.It is very stable molecule.
| Is This Answer Correct ? | 2 Yes | 4 No |
Answer / b.naga vara lakshmi
1.IT SHOW MAXIMUM WAVELENGTH, AND ALSO PRIMARI REFERENCE
STANDARD,
2.IT PHYSICALLY STABLE IN NATURE AND USE LONG TIME,
3.IT IS FREELY SOLUBLE IN ANY SOLVENT.
| Is This Answer Correct ? | 0 Yes | 2 No |
Answer / suresh nv
easily avalable inmarket give good resolution
| Is This Answer Correct ? | 3 Yes | 7 No |
Answer / shivakumar
Caffeine is used for HPLC calibration in detector wavelength
accuracy test just because.
-it is completely soluble in water.
-it have a range of absorption wavelengths.
max at 205nm minima at 245nm and a maxima at 273nm.
- more over it is very stable for long time even in
solution(water).
| Is This Answer Correct ? | 3 Yes | 8 No |
Answer / vikram kodre
along with above answers very important think is caffeine
extracted from nature, so it get in highly pure form.
| Is This Answer Correct ? | 15 Yes | 25 No |
Answer / ajay
caffiene in needle shape easily dissolve in methanol &
methanol is very chip
| Is This Answer Correct ? | 11 Yes | 44 No |
Answer / vaibhav jain
Its somethng chromphore group which is caffeine having i m
nt surely tell bt whnvr i m sure i wl post answer
| Is This Answer Correct ? | 28 Yes | 104 No |
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my name is santhosh i cmpleted b.pharmacy in 2009 then i went to uk in 2010 to pg diploma business management. after i came back to india.. recently again apply to uk for pharmaceutical analysis. they refused my visa you failed to explain y u r change subject. so plz ineed answer for y change the cource
Organic Chemistry 
