Why we use caffeine for HPLC calibration in Detector wave
ength accuracy test

Answer Posted / k,praveen kumar

caffeiene having different wavelents it having 2 maximas
and 1 minima.
1st maxima is 205nm
2nd maxima is 273nm
minima is 245nm
and it is primary reference standard and also suggested in
pharmacopiea.

Is This Answer Correct ?    379 Yes 13 No



Post New Answer       View All Answers


Please Help Members By Posting Answers For Below Questions

What is the Formula for coreletion coefficient in plhplc calibration

1367


How will u perform degradation study in detectors other thann PDA in HPLC Methodology

2106


In Assy by potentio metry titration method how select MET U ,DET U, SET U,

1476


what is the principle involved in turbidometry and nephelometry ?

3733


What is the diffrence in japan mkt requirement in analytical method validation over US?

382






please explian me about area normalisation method,diluted standard method,impurity vs impurity and which one has to be selected in method development

1212


Identify problem faced when mass spectroscopy is used with HPLC system ?

1461


WHAT IS THE PARAMETER FOR THE SELECTION OF MOBILE PHASE IN HPLC?

740


How require to develop GC method? how to select diluent, GAS, column selection and other chromatographic conditions?

382


if your product is soluble in 0.1n hcl and water then which you choose as media from these 2 media?

372


all types of questions asked at the cipla interview

4233


why glutent are detected in the rice cereal baby food product even manufacturer claimed that they are using rice and milk only?we have using ELISA to do the test,and rice supposed not containing any glutent,rite?We already repeat the test so many times and it still detected.just wondering where the glutent came from?

2433


Principle of single pan analytical balance

4690


if tech transfer fails whay require to do?

418


cefoperozone and sulbactam inj. hplc test method

2232