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Why we use caffeine for HPLC calibration in Detector wave
ength accuracy test

Answer Posted / k,praveen kumar

caffeiene having different wavelents it having 2 maximas
and 1 minima.
1st maxima is 205nm
2nd maxima is 273nm
minima is 245nm
and it is primary reference standard and also suggested in
pharmacopiea.

Is This Answer Correct ?    379 Yes 13 No



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