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Why we use caffeine for HPLC calibration in Detector wave
ength accuracy test
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Answer Posted / b.naga vara lakshmi
1.IT SHOW MAXIMUM WAVELENGTH, AND ALSO PRIMARI REFERENCE
STANDARD,
2.IT PHYSICALLY STABLE IN NATURE AND USE LONG TIME,
3.IT IS FREELY SOLUBLE IN ANY SOLVENT.
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2. Two grams of Benzoic acid are dissolved in 200 ml of water and extracted with 200 ml of diethyl ether. The distribution coefficient of benzoic acid is 100, and its dissociation constant is 6.5 10-5. Calculate the distribution ratio (D) of benzoic acid at pH 2, 5, and 6. 3. Calculate D at pH 2 to 10 (1 unit apart) in the above problem, and plot D versus pH.
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