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I want analyze my compound at 325 nm so which standard
shall i use to calibrate my HPLC system? How i can assure
my hplc detector is suitable or not?

Answers were Sorted based on User's Feedback



I want analyze my compound at 325 nm so which standard shall i use to calibrate my HPLC system? Ho..

Answer / jd

For calibration use caffine.
And for hplc detector u calibrate the detector by make 5 lenear solution.And calculate co-relation co-officent it should not less than 0.99

Is This Answer Correct ?    17 Yes 3 No

I want analyze my compound at 325 nm so which standard shall i use to calibrate my HPLC system? Ho..

Answer / aruna kumari

erbium perchlorate solution

Is This Answer Correct ?    4 Yes 0 No

I want analyze my compound at 325 nm so which standard shall i use to calibrate my HPLC system? Ho..

Answer / sandranagarjuna

While we performing wavelength accuracy we can use pyrine as a standard which having absorbable maxima at 318 nm and 333 nm

Is This Answer Correct ?    2 Yes 0 No

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