I want analyze my compound at 325 nm so which standard
shall i use to calibrate my HPLC system? How i can assure
my hplc detector is suitable or not?
Answers were Sorted based on User's Feedback
Answer / jd
For calibration use caffine.
And for hplc detector u calibrate the detector by make 5 lenear solution.And calculate co-relation co-officent it should not less than 0.99
| Is This Answer Correct ? | 17 Yes | 3 No |
Answer / sandranagarjuna
While we performing wavelength accuracy we can use pyrine as a standard which having absorbable maxima at 318 nm and 333 nm
| Is This Answer Correct ? | 2 Yes | 0 No |
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