I want analyze my compound at 325 nm so which standard
shall i use to calibrate my HPLC system? How i can assure
my hplc detector is suitable or not?
Answers were Sorted based on User's Feedback
Answer / jd
For calibration use caffine.
And for hplc detector u calibrate the detector by make 5 lenear solution.And calculate co-relation co-officent it should not less than 0.99
| Is This Answer Correct ? | 17 Yes | 3 No |
Answer / sandranagarjuna
While we performing wavelength accuracy we can use pyrine as a standard which having absorbable maxima at 318 nm and 333 nm
| Is This Answer Correct ? | 2 Yes | 0 No |
what is GLP? Which industries it requried?
1 Answers Aurobindo, AXIS Clinicals, GVK,
what is the different in Total ash, sulphated ash, acid insoluble ash, alcoholic or non alcoholic ash?
why there is a need of optical activity & specific optical activity measurement by polarimeter
How can I develope method of dissolution by HPLC OR UV
Why we are use 0.005M Sulphuric acid, in control of absorbance performance test UV Spectrophotometer calibration
we can use expired sample for validation and analyst qualification?
Why noise test is performed at 254 nm in calibration of HPLC system?
What is the difference between chromatographic purity and related substances?
what is partion and column chromatography
why SGF media used for dissolution . What is the use of it .can we use other media of lower pH instead ot this?
U have 4 strengths have different coating meterial which one u chose to do forced degradation stady in validation
[3/30, 13:29] Manoj P Venkatpurwar: How hplc column selection according to structure? How mobile phase buffer selection on molecule structure?