Why we use caffeine for HPLC calibration in Detector wave
ength accuracy test

Answer Posted / ch prasad drl

1 IT DETECT A MULTIWAVELENGTH 1.205 2. 273 3. 245.
2 AND IT IS PRIMARY REFERENCE STANDARD.
3 IT IS VERY STABLE
4 IT IS VERY EASIY AVAILABLE IN MARCKET
5 IT IS CHEAP.

Is This Answer Correct ?    1 Yes 0 No



Post New Answer       View All Answers


Please Help Members By Posting Answers For Below Questions

in hplc chromatogram started from left to right and in uv spectrum started from left to right

548


What are modes of quantification in related substances

1825


how can I make the copper get white bye any salt or acit?

1762


How do we quantify crystaline and amarpous forms by using (NMR, XRD)spectroscopic techniques? Which any others instruments are useful for this quantification? explain

2043


How to determine water content of bis tetrazole amine mono ammonium salt

3058






Why perchloric acid used for potentiometric titration

2279


what is the different in Total ash, sulphated ash, acid insoluble ash, alcoholic or non alcoholic ash?

9561


What is similarity factor and its use in api strength?

882


My question about gas chromatography sulfur chemiluminsecence detector. I test unknown sample gas by GC-SCD (calibrated ) and the result of *H2S is 279 PPM , *but when I test the same sample with the GC-TCD (calibrated ) the value of *H2S is 2500 PPM . I'd like to inform you that both GCs are calibrated and have very good operation conditions with stable parameters . the question is if the sample gas with higher H2S over detection limits of SCD detector (1000 ppm). why the result it 279 ppm Best regards

1198


Which parameters require to do in tech transfer?

364


Why acetonitrile and water are used as extraction solvent when analysis melamine? I thought they are miscible and won't be able to separate...

1318


What is split ratio in Gc? Splitless? how requirr to select?

422


give clarity of linearity and range in method validation

2016


EXPLAIN THE CALCULATION PART OF AN KF TITRATOR CALIBRATION WITH SUITABLE EXAMPLE

2219


mode of absorption in alimentary canal?

1999