Why we use caffeine for HPLC calibration in Detector wave
ength accuracy test
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Answer Posted / narayanan
# Potasium dichromate in perchloric acid and Halmium oxide
in perchloric acid can be used for calibration.
# But, after calibration it takes long time to get neutral
range ( sinceacidic in nature).
# lot of washing procedure needs to be followed.
# caffeine is water soluble and easily removable.
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