Why we use caffeine for HPLC calibration in Detector wave
ength accuracy test
Answer Posted / surekha
caffiene is available in pure state and it is a primary
standard .
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In HPLC calibration, caffeine is used as primary standard for wave length calibration due to caffeine is having dual maxima at 273 & 205 nm and one minima at 245 nm. Any body can give reference of these details from any pharmacopeia (with chapter no.) or any other authentic guideline?