HOW RRF IS CALCULATED IN HPLC
Answers were Sorted based on User's Feedback
Answer / bujji kanchi
1)inject same concentration of impurity and active
Ratio of impurity area and active area
correct the concentrations.
Best practice is
Inject LOQ to 150% of specification around 5 levels
and calculate the slope of impurity and slope of active
take the slpe ratio.
Regards,
Bujji Kanchi.
Is This Answer Correct ? | 8 Yes | 0 No |
Answer / javed
RRF Can be calculated by comparing the area of standard with
area of impurity x conc of imp by conc of std x 100.
Is This Answer Correct ? | 9 Yes | 8 No |
calibrtion procedue of LC-MS
acetic acid is weak acid & perchloric acid is strong acid.but how perchloric acid is soluble in acetic acid
which are the diffrent batches in the pharmaceuticals?
a bottle of concerntrated HCL (36.5) was labelled as 37% (w/w) and density 1.19m/l.What is hcl molarity?
what are the different methods of analysing related substances test,what are LOD AND LOQ .HOW TO REPORT IMPURITIES WHEN LOD AND LOQ LIMITS R SPECIFIED
WHAT IS USE OF ION PAIR REAGENTS?
what parameters we will consider while developing a HPLC method and how we confirm our HPLC method is valid?
what is the difference between Hydrochloric Acid & Hydrogen chloride
Hello Dear all; I have a technical question related to analytical Chemistry, I have a sample of (40% w/w) Ferric Chloride solution (for water treatment purposes which consist a little portions of Ferrous Chloride and Hydrochloric acid. I need to know the methods to determine the percentages of each Ferrous Chloride and Chloride acid. Also I want to make sure that this solution is truly (40% w/w). So ANY CLUE !!! Thanks in advance.
Why Deuterium lamp is used as UV light Source for UV.
what is the procedure for cleaning of lenses of hatr accesory of ftir instrument?
How can the GC or HPLC method is selected to determine the impurity profile in drug product?