Why we use Uracil for HPLC calibration in Detector wave
ength accuracy test?
Answers were Sorted based on User's Feedback
Answer / poojitha
uracil is a primary standard and shows maximum absorbance
at 260nm
| Is This Answer Correct ? | 30 Yes | 9 No |
Answer / dhayasiva
Uracil is used as void volume indicator, Hence it is not
retained in HPLC column
| Is This Answer Correct ? | 15 Yes | 7 No |
Answer / gangi reddy
Since it is a standard, Its a unretained substance in the
HPLC Column. No column is required for its elution.
| Is This Answer Correct ? | 4 Yes | 4 No |
Answer / mr. bujji reddy kanchi
Since it is a standard, Its a unretained substance in the
HPLC Column. No column is required for its elution.
| Is This Answer Correct ? | 5 Yes | 8 No |
Answer / flamingo rabale adl
1.What is stability indicating assay/ RS method ?
2. What is descriminatory disolution medium ?
| Is This Answer Correct ? | 6 Yes | 23 No |
Dear Sir, This is Thirunavukkarasu am working in Ordain Health Care AR&D, Am Having one problem While doing method development my question Metadoxine and Pyridoxine same chemical nature and structural activity i tried lot of methods but Analyte eluted same Retantion time i cant separate both peaks. solubility, pH both are same only only difference melting point metadoxine 210 C Pyridoxine 102 C please do the help needful for me
If combination product how require to identify which imp is of which api?
difference between HPLC & LC-MS
what is the results if we use ODScolumn instead of BDS column ,(ther parameter is same for column) in assay method ?
What is the limit for single maximum impurity limit as per USP??
stability study is going on up to 3years for famotidine-USP but the pharmacopeia is revised and tlc test is replaced with hplc test. At the 4th year frequency the product is failed in hplc test .how can i assain the expiry or retest date and can i stop or continue the programme?
importance of internal standard and in what conditions it is used?
how to pepare 6.8 pH phosphate buffer
What is the reason for using the 900ml of dissolution medium
difference between AAS & flame photo meter ?
what is the difference between LOSS ON DRYING IN ORDINARY OVEN TECHNIQUE AND VACUUM OVEN TECHNIQUE
what is the difference of use of LOD test and Karl Fischer titra.?