Why we use Uracil for HPLC calibration in Detector wave
ength accuracy test?
Answers were Sorted based on User's Feedback
Answer / poojitha
uracil is a primary standard and shows maximum absorbance
at 260nm
Is This Answer Correct ? | 30 Yes | 9 No |
Answer / dhayasiva
Uracil is used as void volume indicator, Hence it is not
retained in HPLC column
Is This Answer Correct ? | 15 Yes | 7 No |
Answer / gangi reddy
Since it is a standard, Its a unretained substance in the
HPLC Column. No column is required for its elution.
Is This Answer Correct ? | 4 Yes | 4 No |
Answer / mr. bujji reddy kanchi
Since it is a standard, Its a unretained substance in the
HPLC Column. No column is required for its elution.
Is This Answer Correct ? | 5 Yes | 8 No |
Answer / flamingo rabale adl
1.What is stability indicating assay/ RS method ?
2. What is descriminatory disolution medium ?
Is This Answer Correct ? | 6 Yes | 23 No |
HPLC Calibration was done in Reverse Phase, but not in normal phase why explain?
From where require to take the RLD sample?
Retention time & Relative Retention time Definition & between difference? How to Calculate response factor(RF) & relative response factor(RRF)?
Why pH range is 1-14 nt more than 14???
3 Answers IPCA, Lincoln Pharmaceuticals,
What is the difference between BD
What is primary & Secondary Standard ? Give Example
analytical method validation require to with respect to release specification or shelf life specification?
What is the solvent/material used to balance spirit level in analytical balance?
5 Answers Glenmark, MMC Health Care,
same sample on two preapration gives differnt result during hplc analysis
In GC calibration,what is the procedure to calibrate the TCD (thermal counductivity detector)suggest ?
What are the limits for uniformity dosage content for tablets and capsules
what is the difference between the ordinary loss on drying technique and vacuum oven technique?