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HOW RRF IS CALCULATED IN HPLC

Answer Posted / bujji kanchi

1)inject same concentration of impurity and active

Ratio of impurity area and active area

correct the concentrations.


Best practice is

Inject LOQ to 150% of specification around 5 levels

and calculate the slope of impurity and slope of active

take the slpe ratio.



Regards,
Bujji Kanchi.

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