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Why we are injecting low load standard conc. while analysing Related Substances by HPLC?
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Answer / rajkumar
It is the concentration of the Unknown impurity which comes
from the reporting threshold. For any query see ICH Q3B(R2)
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Answer / ravi kumar tummala
yes, we r injecting std injection at low concentrtion,one reason is impurity specification level and another one is to calculate the imp's against std area.
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hii, i'm had a sample of 1L that gone through extraction & evaporation. i used 0.5ml of methanol for reconstitution before injecting the sample to HPLC. i made a calibration curve using a standard solution but now i'm confused about how to display the concentration i got from the sample. for ex. i got 0.2ug/ml how i'm gonna explane this is for the 1L ? please help .. i'll really appreciate it thanks
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