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Why we are injecting low load standard conc. while analysing Related Substances by HPLC?
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Answer / rajkumar
It is the concentration of the Unknown impurity which comes
from the reporting threshold. For any query see ICH Q3B(R2)
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Answer / ravi kumar tummala
yes, we r injecting std injection at low concentrtion,one reason is impurity specification level and another one is to calculate the imp's against std area.
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