Why 10%,20%,30% Sucrose solution is used for the calibration
of polarimeter?
Answers were Sorted based on User's Feedback
Answer / devendra birla syncom formulat
Actually what happened when we use above 30% conc. supposed we using 40% conc of sugar or excess of this the dimerization will occur there means same principle of UV spectroscopy so two molecule arrange in array one behind other in such cases you will got minimum optical rotation in respect to actual concentration of sample your linear curve will not complies in correlation i.e. NLT 0.9995. I think so you got satisfaction by this answer reply me fast
| Is This Answer Correct ? | 22 Yes | 2 No |
for linearity purpose and calculation of correlation
coefficient
| Is This Answer Correct ? | 28 Yes | 11 No |
Answer / naveen reddy
surose is stable optical rotation
above 30% it will not give correct results
so dilute half and measure THETA and multiplie with two
| Is This Answer Correct ? | 15 Yes | 3 No |
What is post run in hplc?
What is the calibration procedure for Malvern MS2000 particle size analyzer and acceptance criteria?
What is the difference between bulk property detectors and Solute property detectors vivimed labs limited
how can standardized of HCl of 0.1 N
What is Spliit and Splitless in Gas Chromatography nad How does it work?
WHAT IS DIFFERENCE B/W MALTINGRANG/DSC
how require to set assay concentration for standard and sample?
why there is a need of optical activity & specific optical activity measurement by polarimeter
we are performed SOR for a particular product the limit is -6 to -10.We face particular bathes it is not meeting the specification.we performed diffrent instrument diffrent analyst we are getting diffrent diffrent results.solvent is dimethyl sulfoxide what could be the reasion
what does 'n' stands for in spectroscopy?give the formula.
How can we confirm the HPLC column is end-capped or not? Is it possible to identify by physical appearance?
why ph is between 0 to 14
6 Answers Apotex, Reliance, Zydus Cadila,