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What is third generation HPLC Columns?
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what is the importance of peak purity in HPLC and how we can calculate through manul(not software)?
we run the same sample in same method in different flow rates in HPLC,any changes in sample analysis?
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What is delay volume?
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how a particular wavelength can be different for a particular compund while analysing by uv and by HPLC.
In C8,C18....etc have 4&5 etc... microns Columns is there like that how to separate Chiral columns as per Micron size?
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Organic Chemistry 
