If compound Pka is 5 then which buffer you will select ?
- 5 Answers
- 15058 Views
- Avra, GVK, I also Faced
- E-Mail Answers
Answers were Sorted based on User's Feedback
Answer / shyam kumar
The thing is u should not use mobile phase pH 5.00. any pH
u can use generally +/- 1.00 unit is preferable. in that
also selection of lowe or higher pH is depending on your
compound nature and noise. in whis pH u have less noise
choose that.
| Is This Answer Correct ? | 19 Yes | 0 No |
Answer / chandu 4m apex
u should take pka = +/-2 (ph)
ex; pka 5 ph should be either 3 or 7
| Is This Answer Correct ? | 10 Yes | 1 No |
Answer / sandip ankolia
u can not say that if Pka is 5 so use buffer near about pka
5 but depends upon the nature of compound , drift and noise.
genrally u can selet pH +/- 1.5 unit of the Pka value of
the compound.
Most important thing is that its not react with buffer,
| Is This Answer Correct ? | 5 Yes | 0 No |
Answer / venkataramireddy.u
Compound pka value is 5 u have to choose pka value 5
buffer and adjust ph + or - 1.5 units and buffer uv cut off
should be below 200 nm,good solubulity with organic
solvents.
| Is This Answer Correct ? | 2 Yes | 0 No |
Answer / u.venkatramireddy
compound nearly containing buffer will be taken like
ammonium acetate pka 4.8 adjusted ph plus or minus 2
units. posted by uvreddy,DR REDDYS
| Is This Answer Correct ? | 4 Yes | 10 No |
what is difference of uv and pda
13 Answers Akums, MSN, MSN Pharma, Shilpa Medicare,
what are the standard methods for HPLC?
how you determine moist.of NaOH sol or NaOH flacks
Is there any difference in principal of reverse phase and normal phase chromatography? and why? Please update. Thank and regard
Why sulfuric acid is used in sulphated ash / residue on ignition test.
9 Answers CS Pharmaceuticals, Dr Reddys, GVK, HPLC, Micro Labs, Samrudh, Watson Pharmaceuticals, Wings Customer Care,
Can you please give a suitable definition and an example for understanding the words purity and potency.
6 Answers Cipla, Malladi Drugs and Pharmaceuticals, Reddy Labs,
what is dissolution
what is difference between IR lamp and polarimeter lamp
Why pyrene is also use for calibration if detector
why we are use metthanol variously in kfr?why don't use other solutions?
in what basis wavelength will select for a compound at initial method development trials
In HPLC what is Single point threshold, peak purity index and minimum peak purity index? what should be consider for pure peak.
Organic Chemistry 
