Answer / shyam kumar

The thing is u should not use mobile phase pH 5.00. any pH
u can use generally +/- 1.00 unit is preferable. in that
also selection of lowe or higher pH is depending on your
compound nature and noise. in whis pH u have less noise
choose that.

Answer / chandu 4m apex

u should take pka = +/-2 (ph)
ex; pka 5 ph should be either 3 or 7

Answer / sandip ankolia

u can not say that if Pka is 5 so use buffer near about pka
5 but depends upon the nature of compound , drift and noise.
genrally u can selet pH +/- 1.5 unit of the Pka value of
the compound.
Most important thing is that its not react with buffer,

Answer / venkataramireddy.u

Compound pka value is 5 u have to choose pka value 5
buffer and adjust ph + or - 1.5 units and buffer uv cut off
should be below 200 nm,good solubulity with organic
solvents.

Answer / u.venkatramireddy

compound nearly containing buffer will be taken like
ammonium acetate pka 4.8 adjusted ph plus or minus 2
units. posted by uvreddy,DR REDDYS

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