Answer / samresh pal

pH Play the role for ionisation and unionisation of drug molucule in mobile phase..if our drug moity is acidic nature and our mobile phase pH is acidic then our drug moity behave to unionised and unionised molecule behave like hydrophobic or nonpolar so drug moiety more attract to stationary phase( coloum) so it not eluted with mobile phase readily and mor time expend in coloum.

Answer / hitesh tank

Specific columns depends on mobile phase pH. Column life's depend on pH it should be 2 to 8 .other wise high pH mobile phase create silanol and silica get dissolved, law pH flushed out stationary phase from the columns.

Answer / hitesh tank

HPLC Analysis depend on pH ,Peak symmetry, retention time, selectivity and impurities detection, RRT,etc.

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