Interested to Buy Any Domain ? << Click Here >> for more details...
how can we determine RRF value in HPLC FOR CALCULATION OF
IMPURITY ?
- 4 Answers
- 50718 Views
- Macleods, I also Faced
- E-Mail Answers
Answers were Sorted based on User's Feedback
RRF-Relative responce factor with respect to analyte(standard)
why its required- due to impurity cost,availability and time
consumption of analysis etc we can not go for everytime
impurity by impurity calculation every time
so,that finding a factor for impurities with respect to
standard.by preparing few levels of(3+ levels)standard and
impurities concentrations(can prepare between +/- 50% of
specification level of known impurities and for standard
between +/- 50% of specification level of unknown
impurities).inject the above prepared solution in to
hplc,calculate slope for impurities and standard by using
respective concentrations and areas.
RRF=SLOPE OF IMPURITIES/SLOPE OF STANDARD
| Is This Answer Correct ? | 55 Yes | 7 No |
Answer / v n murthy
RRF = IMP AREA/STD AREA*STD CONC/IMP CONC*STD PURITY/IMP
PURITY
STD AREA =MAIN PEAK AREA
| Is This Answer Correct ? | 55 Yes | 8 No |
Answer / godhani pragnesh
RRF= Relative response factor (Impurity response with
respact to main peak)
RF= Response factor (the response (e.g. peak area) of drug
substance or related substances per unit weight.
RF= peak area / concentration (mg/ml)
Relative response factor (RRF)calculation:
RRF=RF impurity / RF API
OR
RRF=slope impurity / slope API
| Is This Answer Correct ? | 42 Yes | 6 No |
Answer / vinod
In agilent hplc(ezchrome software ) we go to the report option and then right click ,the option of rrf apper add it ,it automatically apper in ur report
| Is This Answer Correct ? | 8 Yes | 38 No |
Who discovered X-ray
2 Answers Huntsman Corporation,
What is the reason of RT change in new column v/s old column in HPLC
One pH means how much volts and milivolts?
wt is rsd in and explain
why we are performing residue on ignition?
what is difference between zero air gas and o2 gas
1 Answers IPCA, Vasudha Pharma,
Nitrogen test is used for which chromatography?
what is the difference b/w repeatability nd reproducibility ?
IN DISSOLUTION S1 STAGE 1 BOWL GOT 52%(Q POINT IS 80%)WHAT WE HAVE DO
if you get peak in blank then what require to do?
What is FFA, PV, and PAV value in oil.
What is difference b/t uv detector and rid
Organic Chemistry 
