how can we determine RRF value in HPLC FOR CALCULATION OF
IMPURITY ?
Answers were Sorted based on User's Feedback
RRF-Relative responce factor with respect to analyte(standard)
why its required- due to impurity cost,availability and time
consumption of analysis etc we can not go for everytime
impurity by impurity calculation every time
so,that finding a factor for impurities with respect to
standard.by preparing few levels of(3+ levels)standard and
impurities concentrations(can prepare between +/- 50% of
specification level of known impurities and for standard
between +/- 50% of specification level of unknown
impurities).inject the above prepared solution in to
hplc,calculate slope for impurities and standard by using
respective concentrations and areas.
RRF=SLOPE OF IMPURITIES/SLOPE OF STANDARD
Is This Answer Correct ? | 55 Yes | 7 No |
Answer / v n murthy
RRF = IMP AREA/STD AREA*STD CONC/IMP CONC*STD PURITY/IMP
PURITY
STD AREA =MAIN PEAK AREA
Is This Answer Correct ? | 55 Yes | 8 No |
Answer / godhani pragnesh
RRF= Relative response factor (Impurity response with
respact to main peak)
RF= Response factor (the response (e.g. peak area) of drug
substance or related substances per unit weight.
RF= peak area / concentration (mg/ml)
Relative response factor (RRF)calculation:
RRF=RF impurity / RF API
OR
RRF=slope impurity / slope API
Is This Answer Correct ? | 42 Yes | 6 No |
Answer / vinod
In agilent hplc(ezchrome software ) we go to the report option and then right click ,the option of rrf apper add it ,it automatically apper in ur report
Is This Answer Correct ? | 8 Yes | 38 No |
In HPLC Calibration, On which basis RSD Limit of noise test is fixed (NMT 33.0 % )
what is batch no. of prednisole tablet.
What is the difference of time between two consecutive injections in HPLC?
2 Answers Nectar Lifesciences,
Calculate the water content in Di Sodium Tartrate (DST) based on molecular weight?
Why dissolution test performed on six jar?
why you are using benzoic acid for water content determination in TEA
What is the principle of HPLC, GC, LCMS, GCMS,LC QQQ, GC QQQ, LC Q TOF and GC Q TOF. What are the applications and Specificity?
When recording an IR spectrum in the lab, we take no special precautions to account for the presence of oxygen, nitrogen, or argon in the air. What are the consequences of this?
Which Zone of our country like conducting of Stability studies(Accelerated & Long term stability studies)as per ICH guidelines.
why we can use only 4 and 7 and 9 buffers in pH calibration
what type of compounds do we see lod @ wat type compounds do we see m.c
what are the standard methods for HPLC?