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WHAT IS DIFFERENCE BETWEEN RRT AND RRF IN HPLC
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Relative retention time (RRT) is used to know where peaks apart from main compound elutes in HPLC analysis.
Where as Relative Response factor (RRF) comes into picture when our compound and impurities have different wavelength maxima. In this case impurities show either higher or lower response when the analysis is performed at wavelength maxima of main peak. So in quantification analysis the values obtained for impurities are not the actual values. In order to calculate the actual value of impurities' a correction factor is applies. this factor is called RRF
| Is This Answer Correct ? | 84 Yes | 6 No |
Answer / kanakadurga
RRT means Relative retention time. RRF Means Relative
response factor.
Calculate RRT of impurities with main peak RT. Calculate
the RRF with the same peak
response ar 10% level.
| Is This Answer Correct ? | 19 Yes | 24 No |
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