WHAT IS DIFFERENCE BETWEEN RRT AND RRF IN HPLC
Answers were Sorted based on User's Feedback
Relative retention time (RRT) is used to know where peaks apart from main compound elutes in HPLC analysis.
Where as Relative Response factor (RRF) comes into picture when our compound and impurities have different wavelength maxima. In this case impurities show either higher or lower response when the analysis is performed at wavelength maxima of main peak. So in quantification analysis the values obtained for impurities are not the actual values. In order to calculate the actual value of impurities' a correction factor is applies. this factor is called RRF
Is This Answer Correct ? | 83 Yes | 6 No |
Answer / kanakadurga
RRT means Relative retention time. RRF Means Relative
response factor.
Calculate RRT of impurities with main peak RT. Calculate
the RRF with the same peak
response ar 10% level.
Is This Answer Correct ? | 19 Yes | 24 No |
what parameters we will consider while developing a HPLC method and how we confirm our HPLC method is valid?
what is least count of a balance? How we find out this? Any formula for least count of Analytical balance?
13 Answers Acme Formulation, Analytical Solutions, Dr Reddys, Reddy Labs,
Why we use uracil and caffeine in hplc calibration
We use hicrome 10 cxs column in hplc analysis....pls tell me.. what is the meaning of 10 cxs?
Whya the need of Specificity to do in Analytical Method Validation ?
What is the lab normality??
what is method development parameter in HPLC method development
How to choose Normal phase or Reverse Phase HPLC method Conditions for given sample?
Difference between standard operating procedure and standard test procedures in pharmaceutical industry
in DMF having extra impurities and in api COA also having extra imp than USP or BP product then how require to proceed?
when we use capillary and packed columns in gc
how to prepare 5 normality of h2so4 solution