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what is role of pH in mobile phase
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Answer Posted / samresh pal
pH Play the role for ionisation and unionisation of drug molucule in mobile phase..if our drug moity is acidic nature and our mobile phase pH is acidic then our drug moity behave to unionised and unionised molecule behave like hydrophobic or nonpolar so drug moiety more attract to stationary phase( coloum) so it not eluted with mobile phase readily and mor time expend in coloum.
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