Interested to Buy Any Domain ? << Click Here >> for more details...
Dear Sir,
This is Thirunavukkarasu am working in Ordain Health Care
AR&D, Am Having one problem While doing method development
my question Metadoxine and Pyridoxine same chemical nature
and structural activity i tried lot of methods but Analyte
eluted same Retantion time i cant separate both peaks.
solubility, pH both are same only only difference melting
point metadoxine 210 C Pyridoxine 102 C please do the help
needful for me
- 5 Answers
- 9896 Views
- Ordain Solutions, I also Faced
- E-Mail Answers
Answers were Sorted based on User's Feedback
Answer / bpatel1773
Dear ,
I thing you want to isolate both compound , You can try
derivatization method using bezoyl chloride or densyl
chloride . Bezoyl chloride will react with only metadoxine
and it will eluted late on HPLC . This way must work .
Benzoy chloride will react at RT only
| Is This Answer Correct ? | 3 Yes | 0 No |
Answer / a.prabhakar
Dear Thirunavukkarasu,
What is your column flow rate and have you change column flow rate.
| Is This Answer Correct ? | 2 Yes | 1 No |
Answer / manoj kumar vadlamudi, ashla
Dear SIR,
Kindly play with different pH also...
and also try with RID detector....
| Is This Answer Correct ? | 0 Yes | 0 No |
Answer / swathi
Once u can use hilic silica column ..mostly water %dont exceed 20% in gradient program otherwise phenyl column particle size 3micron..slow gradient ...flow rate decrease..and at which they are eluting there keep isocratic for 30 min
Is it the compounds are different?
| Is This Answer Correct ? | 0 Yes | 0 No |
Do you know what Trace metal clean technique is?describe that technique?
1 Answers Mylan, Nirma, Oil and Gas Jobs, Reddy Labs,
wat is reverse phase in hplc
What is differences between the Instrument and Equipment
In which situation we require to analytical method validation of excipient?
IN pH CALIBRATION,CALIBRATION IS DONE FOR pH METER OR ELECTRODE?HOW WILL YOU JUDGE THAT THE METER TO BE CALIBRATED WITH 4,7,9.2 BUFFERS?EXPLAIN TEMPERATURE EFFECTS ON pH.
What is the limit of minimum area in in hplc RS anlysis
HPLC Calibration was done in Reverse Phase, but not in normal phase why explain?
if the purity is 99.9%,is need to do assay?
Please do not join if u got offer in oncotherapies ltd (STRIDESARCOLAB)especially Mr.Mahesh Bhatt team because job is not secure his team and doesn't have confidence on him becareful this offer?
what is the results if we use ODScolumn instead of BDS column ,(ther parameter is same for column) in assay method ?
why salisylic acid not using now days for disdolution calibration?
Home heating oil or furnace oil what is made from? Can (base oil plus diesel/kerosen) = home heating oil?
Organic Chemistry 
