Dear Sir,
This is Thirunavukkarasu am working in Ordain Health Care
AR&D, Am Having one problem While doing method development
my question Metadoxine and Pyridoxine same chemical nature
and structural activity i tried lot of methods but Analyte
eluted same Retantion time i cant separate both peaks.
solubility, pH both are same only only difference melting
point metadoxine 210 C Pyridoxine 102 C please do the help
needful for me
Answers were Sorted based on User's Feedback
Answer / bpatel1773
Dear ,
I thing you want to isolate both compound , You can try
derivatization method using bezoyl chloride or densyl
chloride . Bezoyl chloride will react with only metadoxine
and it will eluted late on HPLC . This way must work .
Benzoy chloride will react at RT only
Is This Answer Correct ? | 3 Yes | 0 No |
Answer / a.prabhakar
Dear Thirunavukkarasu,
What is your column flow rate and have you change column flow rate.
Is This Answer Correct ? | 2 Yes | 1 No |
Answer / manoj kumar vadlamudi, ashla
Dear SIR,
Kindly play with different pH also...
and also try with RID detector....
Is This Answer Correct ? | 0 Yes | 0 No |
Answer / swathi
Once u can use hilic silica column ..mostly water %dont exceed 20% in gradient program otherwise phenyl column particle size 3micron..slow gradient ...flow rate decrease..and at which they are eluting there keep isocratic for 30 min
Is it the compounds are different?
Is This Answer Correct ? | 0 Yes | 0 No |
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