Dear Sir,
This is Thirunavukkarasu am working in Ordain Health Care
AR&D, Am Having one problem While doing method development
my question Metadoxine and Pyridoxine same chemical nature
and structural activity i tried lot of methods but Analyte
eluted same Retantion time i cant separate both peaks.
solubility, pH both are same only only difference melting
point metadoxine 210 C Pyridoxine 102 C please do the help
needful for me
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Answer / bpatel1773
Dear ,
I thing you want to isolate both compound , You can try
derivatization method using bezoyl chloride or densyl
chloride . Bezoyl chloride will react with only metadoxine
and it will eluted late on HPLC . This way must work .
Benzoy chloride will react at RT only
| Is This Answer Correct ? | 3 Yes | 0 No |
Answer / a.prabhakar
Dear Thirunavukkarasu,
What is your column flow rate and have you change column flow rate.
| Is This Answer Correct ? | 2 Yes | 1 No |
Answer / manoj kumar vadlamudi, ashla
Dear SIR,
Kindly play with different pH also...
and also try with RID detector....
| Is This Answer Correct ? | 0 Yes | 0 No |
Answer / swathi
Once u can use hilic silica column ..mostly water %dont exceed 20% in gradient program otherwise phenyl column particle size 3micron..slow gradient ...flow rate decrease..and at which they are eluting there keep isocratic for 30 min
Is it the compounds are different?
| Is This Answer Correct ? | 0 Yes | 0 No |
hii, i'm had a sample of 1L that gone through extraction & evaporation. i used 0.5ml of methanol for reconstitution before injecting the sample to HPLC. i made a calibration curve using a standard solution but now i'm confused about how to display the concentration i got from the sample. for ex. i got 0.2ug/ml how i'm gonna explane this is for the 1L ? please help .. i'll really appreciate it thanks
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