what are the normalisation, internal standard and external
standerd methods in HPLC.
Answers were Sorted based on User's Feedback
Answer / gitush
External standard is a standard that you bought. Internal standard is the one that you tested and compared to external standard, found it at the same or higher purity and use as a standard in your laboratory / company. Normalization is a process of getting the right concentrate calculated based on standard.
Is This Answer Correct ? | 2 Yes | 1 No |
Answer / s.goutham
internal standard method method known amount of standard
was added in sample. External stanadard method standard
eas perform seperately not with sampe. area normalaisation
was injected as such
Is This Answer Correct ? | 1 Yes | 8 No |
why using the ph 4,7,9.2?
acetic acid is weak acid & perchloric acid is strong acid.but how perchloric acid is soluble in acetic acid
What is definition of RRT and what is the difference between RT and RRT in HPLC
1)What's the meaning of Absorption,give a example. 2)What's the meaning of Adsorption,give a example. 2)what is the difference between Absorption and Adsorption.
0 Answers VHB Medi Life Sciences,
how you fix the limits of impurities?
WHICH STANDARD WE USE IN SILICA ANALYSIS IN SILICA-GEL METHOD FOR SOLID SAMPLE
mode of absorption in alimentary canal?
effect of pore size, pore volume, partical size, column length, carbon load on retention time? what is carbon load? what is the use?
How will you select mobile phase in HPLC Method Development
how the compound separate in coulunm,explain
Why 10%,20%,30% Sucrose solution is used for the calibration of polarimeter?
5 Answers Cipla, LGC Promochem,
a bottle of concerntrated HCL (36.5) was labelled as 37% (w/w) and density 1.19m/l.What is hcl molarity?