Generally for Normal Phase HPLC,stationary phase is Polar.In place of polar if

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Generally for Normal Phase HPLC,stationary phase is Polar.In
place of polar if we take non-polar means what happened & why?

Question Posted / siri

7 Answers
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Generally for Normal Phase HPLC,stationary phase is Polar.In place of polar if we take non-polar m..

Answer / santosh

In normal phase , stationary phase is polar while mobile
phase is non polar & separation is achieve by the polarity
of the compound. More polar compound eluted out last while
non polar compound eluted fast

Is This Answer Correct ?

20 Yes

4 No

Generally for Normal Phase HPLC,stationary phase is Polar.In place of polar if we take non-polar m..

Answer / sri

Supose if we take non polar stationary phase and non polar
mobile phase bonded phase will lose.becase non polar
stationary phse dissolved in non polar mobile phase.

Is This Answer Correct ?

11 Yes

0 No

Generally for Normal Phase HPLC,stationary phase is Polar.In place of polar if we take non-polar m..

Answer / umesh patel

in case of normal phase chromatograpy, as we are using
highly non polar sovent as mobile phase ,it will dissolve
the column bed of non polar SP

like dissolves like

Is This Answer Correct ?

7 Yes

3 No

Generally for Normal Phase HPLC,stationary phase is Polar.In place of polar if we take non-polar m..

Answer / nagaraj

in normal phase chromatography is used for seperation of
compounds which are having non polar segments .if we take
non polar asorption can acheived against separtion.because
it is seperation chromatogrphy.

Is This Answer Correct ?

1 Yes

1 No

Generally for Normal Phase HPLC,stationary phase is Polar.In place of polar if we take non-polar m..

Answer / aniket

column will damage &resolution will reduced

Is This Answer Correct ?

0 Yes

0 No

Generally for Normal Phase HPLC,stationary phase is Polar.In place of polar if we take non-polar m..

Answer / ch s raju

in normal phase we are using non polar solvent as a mobile
phase and if we are using non-polar stationary phase it
dissolves in non-polar solvent column collapes

Is This Answer Correct ?

0 Yes

0 No

Generally for Normal Phase HPLC,stationary phase is Polar.In place of polar if we take non-polar m..

Answer / amol

In normal phase , stationary phase is polar while mobile
phase is non polar & separation is achieve by the polarity
of the compound. More polar compound eluted out first while
non polar compound eluted last . if we take non polar
stationary phase it will happen reverse..

Is This Answer Correct ?

5 Yes

20 No

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