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what is reverse phase chromarography&normal phase
chromatography
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Answer / jangam shyam
in revarcephase chromatography we use the non-polar
stationaryphase and polar mobile phase.in this type the
polar analytes elute first,non polar analytes elute later.
in normal phase chromatography the stationaryphase is
polar,and the mobilephase is nonpolar.in this type the non
polar analytes elute first,the polar analytes elute later.
| Is This Answer Correct ? | 26 Yes | 1 No |
Answer / kala
In RPC MOBILE PHASE IS POLAR,STATIONARY PHASE IS
NONPOLAR,FIRST ELUENT IS POLAR.
In NPC MOBILE PHASE IS NONPOLAR,STATIONARY PHASE IS
POLAR,FIRST ELUENT IS NONPOLAR.
| Is This Answer Correct ? | 4 Yes | 0 No |
in reverse phase chromatography we use sp is non ploar,and
mp is polar ,where as in normal phase chromatography we use
sp polar, and mp is non polar,so most of the industries
follow reverse phase chromatography,because mostly organic
compounds are in polar nature and we reduce the Retention
time
| Is This Answer Correct ? | 2 Yes | 0 No |
Answer / guest
in pc stationary phase is polar&mobile phase is non polar.in
which no n
polar compounds eluted first.reverse is reverse phase
| Is This Answer Correct ? | 1 Yes | 8 No |
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