what are the normalisation, internal standard and external
standerd methods in HPLC.

Answer Posted / gitush

External standard is a standard that you bought. Internal standard is the one that you tested and compared to external standard, found it at the same or higher purity and use as a standard in your laboratory / company. Normalization is a process of getting the right concentrate calculated based on standard.

Is This Answer Correct ?    2 Yes 1 No



Post New Answer       View All Answers


Please Help Members By Posting Answers For Below Questions

How to establish relative response factor for hplc. Why it is required?

822


all types of questions asked at the cipla interview

4233


What is third generation HPLC Columns?

1593


why we use glass fiber filters use in some situation?

420


What if impurity area in control sample coming more as compared to LOQ level of impurity ?

1251






which are the sizes of capsules?

504


what is mean by dissolution hydrodynamics?

442


How they found 1mL of K.F reagent is equivalent to 5mg of water and if we change the composition of K.F reagent, is it can neutralize more amount of water?

1403


For limit test of heavy metals in BP, Method C require that the substance is ignited at a temperature not exceeding 800 °C. Why confines such the temperature?

3600


If combination product how require to identify which imp is of which api?

435


How to know the estimated LOQ concentration in ppm

830


In HPLC (Waters) software Empower1,2 &3 what is the updation for each version

2593


acceptance criteria for lod & loq by standard deviation of response and slope??

2207


we can use expired sample for validation and analyst qualification?

407


What is intact assay method development.....?

2107