Answer / rajkumar

In columns Stationary phase is Organic phase is coated over
the Silica surface. But during manufacturing it is not
possiable that all active sites(Silica surface)are coated
with organic phase there will be some vacant areas. In that
areas it is possiable to coat with Long chain Organic phase.
so small chain such CH3 is coated in that areas.

Answer / suresh palacharla

A reversed-phase HPLC column that is end-capped has gone
through a secondary bonding step to cover unreacted silanols
on the silica surface.

Answer / ganesh bhavar

for endcapping in reversed phase column use Trimethylamine
means it protect the OH-group silanol group

what v define from IR & NMR spectroscopy ?

2 Answers   Sun Pharma,

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what is the effect of injection volume calculate flow rate in perkin elmer headspace? (ccm parameter in HS)

1 Answers  

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if rsd failed then what require to do?

0 Answers  

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In method developement or in any analysis if we use small length column for analysis what happen then ? why ?

3 Answers  

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what is DIPE limit in residual solvent?

1 Answers  

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What is use of LOD and LOQ in validtion and give range and in calculation why we use 3.3* LOD here why we use 3.3

2 Answers   Dr Reddys, IPCA, Reddy Labs,

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How can I develope method of dissolution by HPLC OR UV

0 Answers  

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why we wre doing linearity siudiec in hplc method validation

3 Answers   Cipla,

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Definition of beers_ lamberts law

4 Answers   Aarti Industries, Agio Pharmaceuticals, Emcure, Supriya Lifescience,

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for which type of applications gas chromatography is used?

2 Answers  

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what is end capping in hplc coulmn

2 Answers   Lupin, Zydus Cadila,

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stability study is going on up to 3years for famotidine-USP but the pharmacopeia is revised and tlc test is replaced with hplc test. At the 4th year frequency the product is failed in hplc test .how can i assain the expiry or retest date and can i stop or continue the programme?

2 Answers   Nakoda Chemicals,

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