What is the Column Endcaping in HPLC ?
- 3 Answers
- 13748 Views
- Aptuit Laurus, I also Faced
- E-Mail Answers
Answers were Sorted based on User's Feedback
Answer / rajkumar
In columns Stationary phase is Organic phase is coated over
the Silica surface. But during manufacturing it is not
possiable that all active sites(Silica surface)are coated
with organic phase there will be some vacant areas. In that
areas it is possiable to coat with Long chain Organic phase.
so small chain such CH3 is coated in that areas.
| Is This Answer Correct ? | 17 Yes | 1 No |
Answer / suresh palacharla
A reversed-phase HPLC column that is end-capped has gone
through a secondary bonding step to cover unreacted silanols
on the silica surface.
| Is This Answer Correct ? | 10 Yes | 0 No |
Answer / ganesh bhavar
for endcapping in reversed phase column use Trimethylamine
means it protect the OH-group silanol group
| Is This Answer Correct ? | 2 Yes | 4 No |
What is similarity factor?
What is the procedure for UV-spectrophotometer calibration?
Which products will produce when acetic anhydride reacts with Ter-butanol, Sec-butanol, Iso butanol, Dimethylamine etc.
What are the different types of columns used in HPLC
why ur using toulene in hexane in u.v calibration?
3 Answers Biological.e.limited, Cipla,
what are risk assement in the analytical qbd?
what is the difference between isocratic & elution technique?
While performing TOC sst analysis Zero shift disabled & sample analysis zero shift enabled why?
how to selecet an exact coloumn for an new molecule development by hplc how to select exact salt as buffer for new molecule development by hplc what is the the process to select the mode of saparation of compoundes by hplc what is the use of ph of buffer what is use of buffer,ph,organic phase,ans methods how the molecules get saparated in coloumn,
how many types of balance calibration test?
How do you give training to a new chemist
how can we determine RRF value in HPLC FOR CALCULATION OF IMPURITY ?
Organic Chemistry 
