in what basis wavelength will select for a compound at
initial method development trials
Answers were Sorted based on User's Feedback
Answer / ramkrishna thakare
1 ) for any unknown compound firstly solubility check.
2 ) then on the basis of solubility UV - scanning should
be done
3 ) on the basis of UV - scanning Maxima must be
checked.& then on the basis MAXIMA wavelength should be
selected.it may multiwavelength or single-wavelength maxima .
RAMKRISHNA THAKARE -------SANDOZ
| Is This Answer Correct ? | 29 Yes | 1 No |
Answer / u.venkatramireddy
Based on Chromophore in the compound
posted by uvreddy
DrReddy's
| Is This Answer Correct ? | 4 Yes | 2 No |
Answer / vijay
Based on wavelength of the compound. Not only lambda max we have to consider co-elute peak, cut off value of solvent.
| Is This Answer Correct ? | 1 Yes | 0 No |
Answer / shami thakur
Not mentioned type of method development UV or HPLC
During hplc method development mobile phase are selected according to dissociation constant value and all study carried with the help of PDA detector wavelength range 200-400nm
| Is This Answer Correct ? | 1 Yes | 0 No |
In lcms analysis some times we get sodium and potassium adducts. if the compound is havine sodium ions how can we confirm wether sodium is from sample or it is a adduct?
2 Answers Teva Pharmaceuticals,
What is the use of tailing factor ?
I have compare C2H2-air and C2H2-N2O flame AAS on determination calcium. Both use same range of std to plot calibration curve. (2-6ppm) When i measure the sample with phosphate, KCl and LaCl, C2H2-N2O flame give false positive result, around 0.5ppm. When i measure the sample with phosphste, KCl and EDTA. C2H2-N2O flame also give 0.5ppm false positive. But both above mentioned sample would not give false positive when measured by C2H2-air flame. What is the reason?
suppose i give three compounds A,B,C how do u calculate assay of those three componds which method do u prefer
how can you do the analysis of compound given to u by hplc
what parameters we will consider while developing a HPLC method and how we confirm our HPLC method is valid?
what split ratio in gas chromatography.
inhouse product is in capsule form in combination and RLD is in tablet form then can we proceed for multimedia CDP? in inhouse capsule product disso is paddle with sinker in release media is there then RLD product in tablet form then with same as paddle with sinker we can proceed n.a.?
Why base line not start from zero in GC Chromatography analysis.
How can you prepare 0.1% Phosphate buffer solution?
How to calculate the maximum loaded sample concentration in HPLC column?
how to prepare 25 ppm acetone. explian with formula