Answer / ravindra

First take ir of that sample and judge the functional
goroups attached to that compound and also structure of
that compound.
then check solubility.
then UV scan with known concentration and find lymda max.
then selection of column.
first try with regular solvent like water, acn,
methanol.and then go to the buffers of different pH.near to
its pka value.
also different coloumn.

Answer / balaji

first check the structure of the compound.identify the
chromophore groups(for UV Detector),refractive index status
(for RI Detector),reactive status with other chromophore
group compounds(Derivitation method for UV.then check the
solubility status of the compound.prepare a known
concentration solution.

In HPLC select a C18 Column which can serve better for the
polar and not polar compounds.select a buffer pH Oof +/-1
unit of pka value of the compound prepare a buffer mix with
some 20% of organic solvent to chnge the elution early
(based on polarity)

inject the prepared known concentration sample solution in
to above system by observing the chromatogram procced
further by altering buffer
pH,COLUMN,FLOW,TEMPERATURE,ORGANIC RATIO Sample size etc.

but to quantify the unknown concentration we need a known
concentration of standard.

What is the meaning of 624 in DB-624 Capillary column used in GC?

5 Answers   Aurobindo,

---

which brands GC coloumn available in market

4 Answers  

---

What is NPOC method in TOC?

1 Answers   Zydus Cadila,

---

When i inject amines and acid on HPLC then which peak will elut first?

4 Answers   Sun Pharma,

---

why glutent are detected in the rice cereal baby food product even manufacturer claimed that they are using rice and milk only?we have using ELISA to do the test,and rice supposed not containing any glutent,rite?We already repeat the test so many times and it still detected.just wondering where the glutent came from?

0 Answers  

---

what is the general mechanism of high performance liqiud chromatography?

7 Answers   Ajanta Pharma, Amneal Pharmaceuticals,

---

How do you show that equal quanitities of two isomers provide the same response using a PDA

1 Answers  

---

My question about gas chromatography sulfur chemiluminsecence detector. I test unknown sample gas by GC-SCD (calibrated ) and the result of *H2S is 279 PPM , *but when I test the same sample with the GC-TCD (calibrated ) the value of *H2S is 2500 PPM . I'd like to inform you that both GCs are calibrated and have very good operation conditions with stable parameters . the question is if the sample gas with higher H2S over detection limits of SCD detector (1000 ppm). why the result it 279 ppm Best regards

0 Answers  

---

Please tell me about the pH of Polycaboxylic ether is it in the 5-6 range ever or more than 6

0 Answers   Apex Encon Projects Private Limited, Cipla,

---

what is Hilic chromatography?

1 Answers  

---

How to know HPLC column performance?

4 Answers   Meghmani Organics,

---

How can you prepare 0.1% Phosphate buffer solution?

6 Answers  

---