Golgappa.net | Golgappa.org | BagIndia.net | BodyIndia.Com | CabIndia.net | CarsBikes.net | CarsBikes.org | CashIndia.net | ConsumerIndia.net | CookingIndia.net | DataIndia.net | DealIndia.net | EmailIndia.net | FirstTablet.com | FirstTourist.com | ForsaleIndia.net | IndiaBody.Com | IndiaCab.net | IndiaCash.net | IndiaModel.net | KidForum.net | OfficeIndia.net | PaysIndia.com | RestaurantIndia.net | RestaurantsIndia.net | SaleForum.net | SellForum.net | SoldIndia.com | StarIndia.net | TomatoCab.com | TomatoCabs.com | TownIndia.com
Interested to Buy Any Domain ? << Click Here >> for more details...

How we can analysed unknown sample by hplc....

Answer Posted / ravindra

First take ir of that sample and judge the functional
goroups attached to that compound and also structure of
that compound.
then check solubility.
then UV scan with known concentration and find lymda max.
then selection of column.
first try with regular solvent like water, acn,
methanol.and then go to the buffers of different pH.near to
its pka value.
also different coloumn.

Is This Answer Correct ?    2 Yes 0 No



Post New Answer       View All Answers


Please Help Members By Posting Answers For Below Questions

for inorganic molecules require to do RS, Assay and disso?

734


Describe your field experience sample type collected,sample techniques,field measurements taken and equipment used?

2709


How do we fix the sample concentaryion in hplc method development. What is the basis?

1210


how can I make the copper get white bye any salt or acit?

2212


what is partion and column chromatography

2392


Why Ethanol is Used for Standardization of GC Head space?

4231


Why sometime potassium ortho phosphate mix with acetone use for sample preparation for hplc

1471


What is the difference between chromatographic purity and related substances?

1380


What should be the level/ Percentage of Arsenic in Arsenic free zinc.

2233


What is the diference between residual solvents and organic volatile matter

2057


what is the purge flow & how to calculate

2648


If inhouse hplc related substance method is completly diffrent from Usp for finished proďuct with diffrent impurities then how require to prove method equivalecy?

776


what is classification of elemental impurities? what is risk assement in elemental impurities?

760


in sampling of raw material which side is pasted sampled labels

1840


What is control room temperature and which guide line says?

1640