Answer / rc reddy

The question is why linearity in method validation ? In fact
, the linearity is to prove that the method is capable pf
producing the response linearly or proportionally to the
amount present the sample.

Answer / k.praveen kumar macleods pharm

If the method is linear or not from certain range suppose
in assay validation the range is 50% to 150% we are
injecting minimum 6 levels and also Calculate the regration
line the correlation coefficient should be less than 0.999
Here regration line equation is y=mx+c

where y = Regration line
m = Slope
c = Intercept.

and also calculate residual sum of squares from theorical
and practical

Answer / sridhar chinta

Linearity is done in hplc method validation to show that the
method is linear through out the concentration range i.e.
the hplc method can give results according to the set
concentration , for instance if 1x is the area obtained for
50% solution then for 100% solution it should bee 2X i.e.
and so on. So for every hplc method lineariy is a must

what is the difference between system precision and method precision?

3 Answers   Teva Pharmaceuticals,

---

why we want incorporate area of each solution of Intermediate precision and repeatability as well as Accuracy ? What is the use to incorporate area of each solution of Intermediate precision and repeatability as well as Accuracy ?

1 Answers  

---

What does theoretical plates number mean?

1 Answers   Zydus Cadila,

---

what is the conductivity of hplc grade water?

4 Answers  

---

WHEN AN UN KNOWN SAMPLE IS GIVEN TO U,HOW WILL U START THE ANALYSIS(ie FIRST WHICH TEST TO BE DONE,NEXT,THEN NEXT),HOW WILL U DECIDE WHICH TYPE OF ANALYSIS TO BE DONE FOR IT?

2 Answers   Aurobindo,

---

---

What is the column performance and column efficiency?

2 Answers   Reddy Labs,

---

are there in uv lamb that is chractrestic for wavelenghth 205 nm ?????

1 Answers   Beni Suef University, Graviti Pharmaceuticals, Sri Krishna Pharmaceuticals,

---

What is the procedure for the calibration of ph meter?

1 Answers   IBS,

---

difference between pH & pOH ?

4 Answers  

---

How to define samples weight in moisture content

1 Answers  

---

why we are using in ph calibrations ph4,7,9.2 buuffers only?

3 Answers   Reddy Labs, Unichem,

---

What is the purpose of octyl silane columns?

0 Answers  

---