In GC and HPLC ,how we can decide which of following method
will be suitable or use for quantitation of chromatogram at
the time of method development.
1.Area Normalisation
2.External standard method
3.Internal standard method
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Answer / balaji
FOR ASSAY INTERNAL STANDARD METHOD IS GENARALY USED,IF
KNOW CHARETERISED IMPURITIES ARE AVALIBLE GO FOR EXTERNAL
STANDARD METHOD AND NO IMPURITES OR ID STANDARD IMPURITES
AVALIBLE MEANS GO FOR AREA NORMALISATION METHOD
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