tell me about aliphatic acid estimation by hplc which columns can we use
- 1 Answers
- 2839 Views
- Dr Reddys, I also Faced
- E-Mail Answers
Answer / reddy
A colorimetric method for the determination of carboxylic acids based on the dicyclohexylcarbodiimide-coupled reaction of 2-nitrophenylhydrazine and carboxylic acids is described. The product of the reaction is extracted into aqueous sodium hydroxide to produce a blue color. This method is suitable for the analysis of aliphatic acids, but aromatic acids do not react under these conditions.
| Is This Answer Correct ? | 0 Yes | 0 No |
Whya the need of Specificity to do in Analytical Method Validation ?
why we are adjusting the ph in buffer solution preparation
column washing procedure for normal phase and reverse phase?
Tell me something about Vitamin A test method by HPLC
what type of questions asked on analytical balance and also give answers................all pharma companies?
What do you mean by potency in Analytical industry
Ratio of Water and Acetonitrile(diluent) is not recommended to use in cleaning method validation,why?
0 Answers Aurobindo, Reddy Labs,
injection volume accruacy test i calibration of hplc
In UV-VIS spectrophotometer:My diluent is methanol even though i corrected base line for methanol,In my sample scan i found methanol peak.what is the reason?
What should be the calibration procedure for wavelength accuracy in case of HPLC?
significane of detector wavelength accrucy in calibration of hplc
why are using toluene in uv calibraion in resolution performance
Organic Chemistry 
