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How you will select column for HPLC development depends on
nature of compound and how it will work explain in brief?
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Answer / debananda mishra
Polar compounds can be analysed throgh polar stationary
phase and nonpolar compouds can separated through nonpolar
stationary phase but mobile phase used is vice versa.
| Is This Answer Correct ? | 4 Yes | 1 No |
Answer / muzammil thange
Compounds should be seperated on "like likes like basis" ie polar compounds should be separated on polar columns and vice versa. Polar compounds when analysed on C-18 should be treated with modifiers to act like non-polar or neutral compounds.
| Is This Answer Correct ? | 4 Yes | 2 No |
Answer / sarvesh pathak
1.if compound is polar select midpolar or towards the
nonpolar column e.g. C18 is midpolar column which is used.
2.if compound is nonpolar select polar stationary phase
e.g. silica column
3.look for the nature of compound whether it is ionisable
or non-ionisable
| Is This Answer Correct ? | 4 Yes | 6 No |
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