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If we have 120 minutes run time method for HPLC and you wan
to decrease the Run time so what should you do??
- 6 Answers
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Answer / pankaj shrivastava
There are some factors and parameters by which we can
decrease the run time as....
1. by increasing flow Rate
2. by increasing lightly Column oven temperature.
3. by increasing mobile phase strength.
3. by using short length column.
| Is This Answer Correct ? | 28 Yes | 3 No |
Answer / priyanka.r
1)By changing the mobile phase composition of organic solvent(for ex:acetonitrile),then RT will shift.
2)by changing the flow rate, length of the column, temperature.
3)the next option is better choose some advanced Technic like UPLC.
| Is This Answer Correct ? | 13 Yes | 1 No |
1) By increase the mobile phase ratio of organic solvent,
2)By increasing Flow rate, 3)Go for Gradient Progrom 4)
Reduce the column Length And Particle size 5)By Changing the
pH of the mobile phase RT will shift
| Is This Answer Correct ? | 5 Yes | 0 No |
Answer / nirav
By addition of organic solvent in mobile phase, By changing
the flow rate, By changing length of the column.
| Is This Answer Correct ? | 3 Yes | 3 No |
Answer / ra
You must try for UPLC.Or otherwise you have to do hard work on the method.
| Is This Answer Correct ? | 3 Yes | 8 No |
Answer / santhu
BY ADJUSTING THE PH OF THE MOBILE PHASE WITH BUFFERS
| Is This Answer Correct ? | 1 Yes | 7 No |
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