If we have 120 minutes run time method for HPLC and you wan
to decrease the Run time so what should you do??
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Answer Posted / c.thirunavukkarasu
1) By increase the mobile phase ratio of organic solvent,
2)By increasing Flow rate, 3)Go for Gradient Progrom 4)
Reduce the column Length And Particle size 5)By Changing the
pH of the mobile phase RT will shift
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