Golgappa.net | Golgappa.org | BagIndia.net | BodyIndia.Com | CabIndia.net | CarsBikes.net | CarsBikes.org | CashIndia.net | ConsumerIndia.net | CookingIndia.net | DataIndia.net | DealIndia.net | EmailIndia.net | FirstTablet.com | FirstTourist.com | ForsaleIndia.net | IndiaBody.Com | IndiaCab.net | IndiaCash.net | IndiaModel.net | KidForum.net | OfficeIndia.net | PaysIndia.com | RestaurantIndia.net | RestaurantsIndia.net | SaleForum.net | SellForum.net | SoldIndia.com | StarIndia.net | TomatoCab.com | TomatoCabs.com | TownIndia.com
Interested to Buy Any Domain ? << Click Here >> for more details...

If we have 120 minutes run time method for HPLC and you wan
to decrease the Run time so what should you do??

Answer Posted / priyanka.r

1)By changing the mobile phase composition of organic solvent(for ex:acetonitrile),then RT will shift.
2)by changing the flow rate, length of the column, temperature.
3)the next option is better choose some advanced Technic like UPLC.

Is This Answer Correct ?    13 Yes 1 No



Post New Answer       View All Answers


Please Help Members By Posting Answers For Below Questions

What is the difference between chromatographic purity and related substances?

1453


In HPLC calibration, caffeine is used as primary standard for wave length calibration due to caffeine is having dual maxima at 273 & 205 nm and one minima at 245 nm. Any body can give reference of these details from any pharmacopeia (with chapter no.) or any other authentic guideline?

2015


which one is better separation in high pressure liquide chromatogram orlowpressure chromato gram in hplc

2211


In HPLC Calibration, On which basis RSD Limit of noise test is fixed (NMT 33.0 % )

3550


Related substance method equivalency on control sample or spiked sample?

820


analytical method validation require to with respect to release specification or shelf life specification?

847


A ha tc is actual defination of retention time?

1662


In the isomers,enantiomers give the only physical properties present such that no chemical properties present.

2260


iam usig ph buffers merk. manually how to prepare ?

2324


WHAT IS THE PURPOSE WE USE HCL, CHOPPER, BURNER, MONOCHROMATOR in AAS? PLEASE EXPLAIN BRIEFLY

2663


why we are using benzene, anyline in acetic anhdride assay titration method?

2167


EXPLAIN THE CALCULATION PART OF AN KF TITRATOR CALIBRATION WITH SUITABLE EXAMPLE

2685


Which type of column should i use to check the purity of high molecular weight protein using HPLC reverse phase column chromatography? Hi everyone. I wanna to check the purity of high molecular weight protein (collagen) with MW of ~130 kDa using a HPLC. I know C18

1964


If vendor having more imp than monograph then how to proceed? and how to set spec?

1002


in monograph 7 imp are there but in vendor showing 5 imp do not req to include 2 imp for that what will be justification?

781