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why buffer is added in solvent system in HPLC ??

Answers were Sorted based on User's Feedback



why buffer is added in solvent system in HPLC ??..

Answer / balu

I thing to maintain the pH of solution

Is This Answer Correct ?    30 Yes 2 No

why buffer is added in solvent system in HPLC ??..

Answer / t reddy

buffer is used to keep PH stable in ionizable condition.

Is This Answer Correct ?    6 Yes 0 No

why buffer is added in solvent system in HPLC ??..

Answer / haribabu

in Reverse phase chromatography for good resolution

Is This Answer Correct ?    5 Yes 2 No

why buffer is added in solvent system in HPLC ??..

Answer / msreddyvelagala

in solute we may have different products,all these cannot elute at same concetration of mobilephase in short time.solvent is polar,to get the eluates faster we have tochange the mobilephase composition(i.e polarity to be increased up to certain time intervel)

Is This Answer Correct ?    3 Yes 1 No

why buffer is added in solvent system in HPLC ??..

Answer / sasibhushanreddy

to control the ph of the soultion

Is This Answer Correct ?    4 Yes 3 No

why buffer is added in solvent system in HPLC ??..

Answer / vikasnautiyal4

because solvent system intrect
with mixture (they are mixture
of acid and base) to prevent
tha ionisation and controle
ph of analyte.

Is This Answer Correct ?    0 Yes 0 No

why buffer is added in solvent system in HPLC ??..

Answer / vijay bhosale

To maintain the PH oo solution.

Is This Answer Correct ?    0 Yes 0 No

why buffer is added in solvent system in HPLC ??..

Answer / manisha dawale

To prevent ionization,to control retension and for proper peak shape

Is This Answer Correct ?    0 Yes 0 No

why buffer is added in solvent system in HPLC ??..

Answer / tapan shaw

To simulate the dissolution behavior in our body fluid
because our body fluid where drugs release occur is either
acidic or basic.

Is This Answer Correct ?    1 Yes 3 No

why buffer is added in solvent system in HPLC ??..

Answer / ankita

to prevent ionization of weak acids and weak bases.

Is This Answer Correct ?    2 Yes 6 No

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