why buffer is added in solvent system in HPLC ??
Answers were Sorted based on User's Feedback
Answer / t reddy
buffer is used to keep PH stable in ionizable condition.
| Is This Answer Correct ? | 6 Yes | 0 No |
Answer / haribabu
in Reverse phase chromatography for good resolution
| Is This Answer Correct ? | 5 Yes | 2 No |
Answer / msreddyvelagala
in solute we may have different products,all these cannot elute at same concetration of mobilephase in short time.solvent is polar,to get the eluates faster we have tochange the mobilephase composition(i.e polarity to be increased up to certain time intervel)
| Is This Answer Correct ? | 3 Yes | 1 No |
Answer / vikasnautiyal4
because solvent system intrect
with mixture (they are mixture
of acid and base) to prevent
tha ionisation and controle
ph of analyte.
| Is This Answer Correct ? | 0 Yes | 0 No |
Answer / manisha dawale
To prevent ionization,to control retension and for proper peak shape
| Is This Answer Correct ? | 0 Yes | 0 No |
Answer / tapan shaw
To simulate the dissolution behavior in our body fluid
because our body fluid where drugs release occur is either
acidic or basic.
| Is This Answer Correct ? | 1 Yes | 3 No |
How to calculate LOD & LOQ in HPLC Validation Method
14 Answers Dr Reddys, GSK GlaxoSmithKline, Reddy Labs, Reliance, UIS,
Why forced degradation studies are performed in hplc method validation?
In the gas chromatography what is used carrier gas and make up gas?
According to ICH,India is coming under which climatic zones?
why kf factor coming 0.5
why we are taking 89ml concentrated HCL for preparing 1N HCL
In gas chromatographic analysis sometimes we use headspace, why it is required instead of manually injection?
Why KBr is used in FTIR ?
Why we perform the ir analysis?
How to compare XRD graphs against standard and carry polymorpism study of API's by powder XRD method?
why phenolpthalin gives pink colour when we titrate aganist sodium hydroxcide & phenolpthalin gives colourless titrate aganist hydrochlric acid which is un burate
What is the use of tailing factor ?