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why buffer is added in solvent system in HPLC ??
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Answer / t reddy
buffer is used to keep PH stable in ionizable condition.
| Is This Answer Correct ? | 6 Yes | 0 No |
Answer / haribabu
in Reverse phase chromatography for good resolution
| Is This Answer Correct ? | 5 Yes | 2 No |
Answer / msreddyvelagala
in solute we may have different products,all these cannot elute at same concetration of mobilephase in short time.solvent is polar,to get the eluates faster we have tochange the mobilephase composition(i.e polarity to be increased up to certain time intervel)
| Is This Answer Correct ? | 3 Yes | 1 No |
Answer / vikasnautiyal4
because solvent system intrect
with mixture (they are mixture
of acid and base) to prevent
tha ionisation and controle
ph of analyte.
| Is This Answer Correct ? | 0 Yes | 0 No |
Answer / manisha dawale
To prevent ionization,to control retension and for proper peak shape
| Is This Answer Correct ? | 0 Yes | 0 No |
Answer / tapan shaw
To simulate the dissolution behavior in our body fluid
because our body fluid where drugs release occur is either
acidic or basic.
| Is This Answer Correct ? | 1 Yes | 3 No |
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