why buffer is added in solvent system in HPLC ??

Answers were Sorted based on User's Feedback



why buffer is added in solvent system in HPLC ??..

Answer / balu

I thing to maintain the pH of solution

Is This Answer Correct ?    30 Yes 2 No

why buffer is added in solvent system in HPLC ??..

Answer / t reddy

buffer is used to keep PH stable in ionizable condition.

Is This Answer Correct ?    6 Yes 0 No

why buffer is added in solvent system in HPLC ??..

Answer / haribabu

in Reverse phase chromatography for good resolution

Is This Answer Correct ?    5 Yes 2 No

why buffer is added in solvent system in HPLC ??..

Answer / msreddyvelagala

in solute we may have different products,all these cannot elute at same concetration of mobilephase in short time.solvent is polar,to get the eluates faster we have tochange the mobilephase composition(i.e polarity to be increased up to certain time intervel)

Is This Answer Correct ?    3 Yes 1 No

why buffer is added in solvent system in HPLC ??..

Answer / sasibhushanreddy

to control the ph of the soultion

Is This Answer Correct ?    4 Yes 3 No

why buffer is added in solvent system in HPLC ??..

Answer / vikasnautiyal4

because solvent system intrect
with mixture (they are mixture
of acid and base) to prevent
tha ionisation and controle
ph of analyte.

Is This Answer Correct ?    0 Yes 0 No

why buffer is added in solvent system in HPLC ??..

Answer / vijay bhosale

To maintain the PH oo solution.

Is This Answer Correct ?    0 Yes 0 No

why buffer is added in solvent system in HPLC ??..

Answer / manisha dawale

To prevent ionization,to control retension and for proper peak shape

Is This Answer Correct ?    0 Yes 0 No

why buffer is added in solvent system in HPLC ??..

Answer / tapan shaw

To simulate the dissolution behavior in our body fluid
because our body fluid where drugs release occur is either
acidic or basic.

Is This Answer Correct ?    1 Yes 3 No

why buffer is added in solvent system in HPLC ??..

Answer / ankita

to prevent ionization of weak acids and weak bases.

Is This Answer Correct ?    2 Yes 6 No

Post New Answer

More Analytical Chemistry Interview Questions

What is Specificity ?

8 Answers   Dr Reddys,


what is difference of uv and pda

13 Answers   Akums, MSN, MSN Pharma, Shilpa Medicare,


hi i am m.sc organic chem and complete analytical lab tech dipolma now job in qc many time ask question you in m.sc organic why job in qc please help me i can not give proper ans please tell me

3 Answers   Arch Pharmalabs, Natco,


is it nessesary to do solution stability for 7 days?

0 Answers  


why we have to store the pH electrode in 4.01 buffer ?

1 Answers   Aurobindo, BioMax, BioSynth, Cipla, Indoco Remedies, SGS,






which are the guidelines for force degradation studies?

1 Answers  


Why Peak Purity is not determined in GC analysis?

1 Answers   GSFC, GVK,


Why 10%,20%,30% Sucrose solution is used for the calibration of polarimeter?

5 Answers   Cipla, LGC Promochem,


What is principle of Polarimeter?

21 Answers   Intas,


if peak get problamatic then what require to do?

0 Answers  


why ph electrode is storing in KCL solution?Why the pH max is 14.0?

5 Answers   Cadila, Vimta Labs,


analytical method validation require to with respect to release specification or shelf life specification?

0 Answers  


Categories
  • Organic Chemistry Interview Questions Organic Chemistry (342)
  • Inorganic Chemistry Interview Questions Inorganic Chemistry (123)
  • Analytical Chemistry Interview Questions Analytical Chemistry (1387)
  • Physical Chemistry Interview Questions Physical Chemistry (62)
  • General Chemistry Interview Questions General Chemistry (478)
  • Chemistry AllOther Interview Questions Chemistry AllOther (190)