why buffer is added in solvent system in HPLC ??
Answers were Sorted based on User's Feedback
Answer / t reddy
buffer is used to keep PH stable in ionizable condition.
Is This Answer Correct ? | 6 Yes | 0 No |
Answer / haribabu
in Reverse phase chromatography for good resolution
Is This Answer Correct ? | 5 Yes | 2 No |
Answer / msreddyvelagala
in solute we may have different products,all these cannot elute at same concetration of mobilephase in short time.solvent is polar,to get the eluates faster we have tochange the mobilephase composition(i.e polarity to be increased up to certain time intervel)
Is This Answer Correct ? | 3 Yes | 1 No |
Answer / vikasnautiyal4
because solvent system intrect
with mixture (they are mixture
of acid and base) to prevent
tha ionisation and controle
ph of analyte.
Is This Answer Correct ? | 0 Yes | 0 No |
Answer / manisha dawale
To prevent ionization,to control retension and for proper peak shape
Is This Answer Correct ? | 0 Yes | 0 No |
Answer / tapan shaw
To simulate the dissolution behavior in our body fluid
because our body fluid where drugs release occur is either
acidic or basic.
Is This Answer Correct ? | 1 Yes | 3 No |
What is Specificity ?
what is difference of uv and pda
13 Answers Akums, MSN, MSN Pharma, Shilpa Medicare,
hi i am m.sc organic chem and complete analytical lab tech dipolma now job in qc many time ask question you in m.sc organic why job in qc please help me i can not give proper ans please tell me
3 Answers Arch Pharmalabs, Natco,
is it nessesary to do solution stability for 7 days?
why we have to store the pH electrode in 4.01 buffer ?
1 Answers Aurobindo, BioMax, BioSynth, Cipla, Indoco Remedies, SGS,
which are the guidelines for force degradation studies?
Why Peak Purity is not determined in GC analysis?
Why 10%,20%,30% Sucrose solution is used for the calibration of polarimeter?
5 Answers Cipla, LGC Promochem,
What is principle of Polarimeter?
if peak get problamatic then what require to do?
why ph electrode is storing in KCL solution?Why the pH max is 14.0?
analytical method validation require to with respect to release specification or shelf life specification?