why buffer is added in solvent system in HPLC ??

Answers were Sorted based on User's Feedback



why buffer is added in solvent system in HPLC ??..

Answer / balu

I thing to maintain the pH of solution

Is This Answer Correct ?    30 Yes 2 No

why buffer is added in solvent system in HPLC ??..

Answer / t reddy

buffer is used to keep PH stable in ionizable condition.

Is This Answer Correct ?    6 Yes 0 No

why buffer is added in solvent system in HPLC ??..

Answer / haribabu

in Reverse phase chromatography for good resolution

Is This Answer Correct ?    5 Yes 2 No

why buffer is added in solvent system in HPLC ??..

Answer / msreddyvelagala

in solute we may have different products,all these cannot elute at same concetration of mobilephase in short time.solvent is polar,to get the eluates faster we have tochange the mobilephase composition(i.e polarity to be increased up to certain time intervel)

Is This Answer Correct ?    3 Yes 1 No

why buffer is added in solvent system in HPLC ??..

Answer / sasibhushanreddy

to control the ph of the soultion

Is This Answer Correct ?    4 Yes 3 No

why buffer is added in solvent system in HPLC ??..

Answer / vikasnautiyal4

because solvent system intrect
with mixture (they are mixture
of acid and base) to prevent
tha ionisation and controle
ph of analyte.

Is This Answer Correct ?    0 Yes 0 No

why buffer is added in solvent system in HPLC ??..

Answer / vijay bhosale

To maintain the PH oo solution.

Is This Answer Correct ?    0 Yes 0 No

why buffer is added in solvent system in HPLC ??..

Answer / manisha dawale

To prevent ionization,to control retension and for proper peak shape

Is This Answer Correct ?    0 Yes 0 No

why buffer is added in solvent system in HPLC ??..

Answer / tapan shaw

To simulate the dissolution behavior in our body fluid
because our body fluid where drugs release occur is either
acidic or basic.

Is This Answer Correct ?    1 Yes 3 No

why buffer is added in solvent system in HPLC ??..

Answer / ankita

to prevent ionization of weak acids and weak bases.

Is This Answer Correct ?    2 Yes 6 No

Post New Answer

More Analytical Chemistry Interview Questions

what is different between volumetric solution and general solution

1 Answers  


what is the difference between M.C and LOD

21 Answers   Aurobindo, Cipla, Laurus, Matrix, Synthokem Labs,


What is difference between Related Substance & Impurity Profile?

2 Answers   Jubilant Organosys,


what is mean by 21 CFR PART? In that what is 21 ?

1 Answers  


Which products will produce when acetic anhydride reacts with Ter-butanol, Sec-butanol, Iso butanol, Dimethylamine etc.

0 Answers  






using gradient pressure in gas chromatography are not ?using gradient pressure why

0 Answers   Reliance,


for which type of applications gas chromatography is used?

2 Answers  


in which situation require to take incident in validation?

0 Answers  


How require to perform linearity as per ANVISA? What are the acceptance criteria?

0 Answers  


4. Describe the operation of the Craig apparatus. Chapter.3 Equilibrium Processes in Separations

0 Answers  


How to choose concentration of LOQ solutions for RS analysis? Example.

1 Answers   Abbott, Indoco Remedies,


why r u using hplc grade solvents

4 Answers  


Categories
  • Organic Chemistry Interview Questions Organic Chemistry (342)
  • Inorganic Chemistry Interview Questions Inorganic Chemistry (123)
  • Analytical Chemistry Interview Questions Analytical Chemistry (1387)
  • Physical Chemistry Interview Questions Physical Chemistry (62)
  • General Chemistry Interview Questions General Chemistry (478)
  • Chemistry AllOther Interview Questions Chemistry AllOther (190)