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What should be the calibration procedure for wavelength
accuracy in case of HPLC?
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Answer / ankama rao.puppala
wavelength accuracy was done by the following reagents
1.Caffeine
2.Erbium Perchlorate
3.benzophenone
In case of Caffeine
we check the absorbance(Area)the following nanometers
200nm-210nm(205nm is the maximum absorbance)
270nm-280nm(273nm is the maximum absorbance)
240nm-250nm(245nm is the miminum absorbance)
we got the above parameters wavelength accuracy was
passed...
In the above Caffeine was best reagent becos of
it has Two maxima(205nm, 273nm) and one minima(245nm)
More stable, Cheaf cost more over it is recomanded by
Pharmacopeia.
| Is This Answer Correct ? | 17 Yes | 0 No |
Answer / s.elumalai
Normally Above 240 nm Holmium Perchlorate and below 240nm
Caffeine solution are used,There are Holmium perchlorate
filters are available for waters HPLC.Inbuilt wavelength
tests are available for agilent.
| Is This Answer Correct ? | 2 Yes | 1 No |
Answer / aslam
Gemerally 20 ppm solution of URACIL is used for wavelength
calibration at the wavelength rangning from 250nm---252nm---
--254nm-------------------------- upto 262nm to get the
linearity
| Is This Answer Correct ? | 2 Yes | 1 No |
Answer / alpesh patel
Can you give any reference from pharmacopoeia? with chapter number...
| Is This Answer Correct ? | 0 Yes | 0 No |
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