What should be the calibration procedure for wavelength
accuracy in case of HPLC?
Answers were Sorted based on User's Feedback
Answer / ankama rao.puppala
wavelength accuracy was done by the following reagents
1.Caffeine
2.Erbium Perchlorate
3.benzophenone
In case of Caffeine
we check the absorbance(Area)the following nanometers
200nm-210nm(205nm is the maximum absorbance)
270nm-280nm(273nm is the maximum absorbance)
240nm-250nm(245nm is the miminum absorbance)
we got the above parameters wavelength accuracy was
passed...
In the above Caffeine was best reagent becos of
it has Two maxima(205nm, 273nm) and one minima(245nm)
More stable, Cheaf cost more over it is recomanded by
Pharmacopeia.
Is This Answer Correct ? | 17 Yes | 0 No |
Answer / s.elumalai
Normally Above 240 nm Holmium Perchlorate and below 240nm
Caffeine solution are used,There are Holmium perchlorate
filters are available for waters HPLC.Inbuilt wavelength
tests are available for agilent.
Is This Answer Correct ? | 2 Yes | 1 No |
Answer / aslam
Gemerally 20 ppm solution of URACIL is used for wavelength
calibration at the wavelength rangning from 250nm---252nm---
--254nm-------------------------- upto 262nm to get the
linearity
Is This Answer Correct ? | 2 Yes | 1 No |
Answer / alpesh patel
Can you give any reference from pharmacopoeia? with chapter number...
Is This Answer Correct ? | 0 Yes | 0 No |
What is the principle of HPLC?
Retention time & Relative Retention time Definition & between difference? How to Calculate response factor(RF) & relative response factor(RRF)?
wat is reverse phase in hplc
Give me details about dissolution , with name ?
Why monograph of hplc coming upperside of drift but in IR monograph coming in drift side?
Why 1.2% KCl is used for limit of stray light? and why limit of stray light is required in UV calibration?
why you are using benzoic acid for water content determination in TEA
difference between IR& FTIR
Ph meter can show more than 14 ph reading? Why ph range in between 1 to 14 only?
if partition coefficient value was positive means, what it indicates ?
why we use 0.1% acetone in gradient calibration of HPLC?
3 Answers Cipla, Dr Reddys, FDC,
what is mass spectra ? why it is necessary ?