What should be the calibration procedure for wavelength
accuracy in case of HPLC?

Answer Posted / aslam

Gemerally 20 ppm solution of URACIL is used for wavelength
calibration at the wavelength rangning from 250nm---252nm---
--254nm-------------------------- upto 262nm to get the
linearity

Is This Answer Correct ?    2 Yes 1 No



Post New Answer       View All Answers


Please Help Members By Posting Answers For Below Questions

why we are using hexane in calibration of number of drop per mL

1172


how require to interprit the method precision data of hptlc?

299


In Dissolution Test why limit is define Q+5% what is the role of +5%.

2576


How doing qbd practically?

434


in monograph 7 imp are there but in vendor showing 5 imp do not req to include 2 imp for that what will be justification?

267






why octanol used to determine the partition coefficient ?

1920


In Assy by potentio metry titration method how select MET U ,DET U, SET U,

1316


In performance Check of GC Why Hexadecane Peak is Considered 

2557


[3/28, 20:52] Manoj P Venkatpurwar: how many impurities require to inject in assay specificity that how we can find out? and in Rs also how?

353


on the basis of bcs class how to proceed the solubility?

285


how to calculate elemental impurities?

549


In which situation we require to analytical method validation of excipient?

298


what is the principle involved in turbidometry and nephelometry ?

3634


What is the calibration of uv process and preparation

1510


analytical method validation require to with respect to release specification or shelf life specification?

284