what is end capping in hplc coulmn
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Answer / kiran ambekar
Capping of exposed silanol with short chain HC chain
ie.Trimethyl chlorosilane.
Now a day all columns are end capping.
generally End capping column is used for basic compounds
for reducing the peak tailing
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Answer / r.kadambavadani
In chromatography, endcapping refers to the replacement of accessible silanol groups in a bonded stationary phase by trimethylsilyl groups.
Endcapping technology prevents the tailing of a polar compound's peak and shows very high durability even with an alkaline mobile phase because of the strong film covering the stationary phase surface.
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