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what is end capping in hplc coulmn
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Answer / kiran ambekar
Capping of exposed silanol with short chain HC chain
ie.Trimethyl chlorosilane.
Now a day all columns are end capping.
generally End capping column is used for basic compounds
for reducing the peak tailing
| Is This Answer Correct ? | 23 Yes | 3 No |
Answer / r.kadambavadani
In chromatography, endcapping refers to the replacement of accessible silanol groups in a bonded stationary phase by trimethylsilyl groups.
Endcapping technology prevents the tailing of a polar compound's peak and shows very high durability even with an alkaline mobile phase because of the strong film covering the stationary phase surface.
| Is This Answer Correct ? | 1 Yes | 0 No |
Dear sir, This is R.Kadambavadani working in a pharma industry. while doing RS analysis of ibuprofen API, unknown peak eluting some times in the 0.6 Min RT. Kindly send me what is the exact reason behind that. also am using ACN the mobile phase.
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