Interested to Buy Any Domain ? << Click Here >> for more details...
what is end capping in hplc coulmn
- 2 Answers
- 13154 Views
- Lupin, Zydus Cadila, I also Faced
- E-Mail Answers
Answers were Sorted based on User's Feedback
Answer / kiran ambekar
Capping of exposed silanol with short chain HC chain
ie.Trimethyl chlorosilane.
Now a day all columns are end capping.
generally End capping column is used for basic compounds
for reducing the peak tailing
| Is This Answer Correct ? | 23 Yes | 3 No |
Answer / r.kadambavadani
In chromatography, endcapping refers to the replacement of accessible silanol groups in a bonded stationary phase by trimethylsilyl groups.
Endcapping technology prevents the tailing of a polar compound's peak and shows very high durability even with an alkaline mobile phase because of the strong film covering the stationary phase surface.
| Is This Answer Correct ? | 1 Yes | 0 No |
how can we decide the limit of cleaning validation? What is the criteria for it?
significane of detector wavelength accrucy in calibration of hplc
which chromatographic technique is used for softening hard water?
How is the Flame test performed?
Why use the Omeprazole as a reference standard to estimate the assay of esomeprazole by HPLC method
what is diffrence between Specific optical rotation, optical rotation?
key components in hplc?
How to calculate LOD & LOQ in HPLC Validation Method
14 Answers Dr Reddys, GSK GlaxoSmithKline, Reddy Labs, Reliance, UIS,
In the test for control of absorbance in UV calibration why do we use only potassium dichromate and what is purpose of taking a specified amount of 57.0-63.0mg?
How can we calculate "confidence interval" in analytical method validation? Pl. explain with example.
why you require QA approval & what is its importance?
why we are using buffer solutionas as a mobile phase in hplc.
Organic Chemistry 
