Interested to Buy Any Domain ? << Click Here >> for more details...
What is calibration procedure of gredient HPLC pump
- 4 Answers
- 25430 Views
- I also Faced
- E-Mail Answers
Answers were Sorted based on User's Feedback
Answer / abhijeet gavali
You have two different methods for it
1.0 Use Propyl parabean in methanol and pure methanol
2.0 use 0.05% solution of acetone in water and water
| Is This Answer Correct ? | 14 Yes | 3 No |
Mobile phase A is 5.6mg/l of propylparaben in methanol (port
A and B)
Mobile phase B is 100% methanol (port C and D)
Run the test for 20 min by using curve 11 method
sample is Methanol
Acceptance criteria:
A/B/C is between 9.5 % to 10.5
A/B/D is between 9.5 % to 10.5
| Is This Answer Correct ? | 13 Yes | 5 No |
Answer / kuchela rao athota
in waters gradient program
TIME PUMPA% pUMPB% PUMPC% PUMPD% CURVE
0.00 50% 50% 0 0
2.00 0% 0% 50 50 11
6.00 50% 50% 0% 0 11
10.00 45% 45% 10 0 11
12.00 50% 50% 0 0% 11
14.00 45% 45% 0 10 11
16.00 50% 50% 0 0 11
18.00 50% 50% 0 0 11
| Is This Answer Correct ? | 8 Yes | 6 No |
Answer / prerna
Gradient Programme for port A and C :
00 : 100
00 : 100
25 : 75
25 : 75
50 : 50
50 : 50
75 : 25
75 : 25
100 : 00
100 : 00
Above programme also run for port B and D
An increasing stepwise piramid will appear. Integrate that
peak and also split each step of the peak.
Find out its height and area of height
By using the formula ( which I don't remeber ), mean flow
of solvent at each step.
Flow : Limit
25 (24-26)
50 (49-51)
75 (74-76)
100 (100)
If the observed value is within the acceptance limit then
that pump passes the test.
| Is This Answer Correct ? | 10 Yes | 10 No |
why 0.1M OR 1M Perchloric acid standardisation done after 24 hours?
state the principle of gas chromatography?
Why forced Digradition Study is done
Optical Rotation*0.9477*100*200] Assay Of dextrose= ________________________________ 99.8* 200 why Factor=0.9477
1 Answers Claris Lifesciences, Inven Pharmaceuticals, Levo,
what is custom fields in waters system?
1 Answers Akorn India, Aurobindo,
What is difference between Chromatographic purity and Related substances analysis?
How we develop a method by HPLC for unknown compound,if we got three peaks with same response then how we can find out which one is our desired compound's peak without taking help of LC MS.
In dissolution test s1 stage we are getting one bowl 40 % out of 6 bowls what is the root cause you find and how proceed next step? ( Q is 80%)
Why methanol & acetonitrile is mostly used for hplc analysis?
What is the deference between Residual solvents and OVI
why in GC calibration of linearity regression coefficient should not be less than 0.999
What is heavy water
Organic Chemistry 
