Interested to Buy Any Domain ? << Click Here >> for more details...
What is calibration procedure of gredient HPLC pump
- 4 Answers
- 25817 Views
- I also Faced
- E-Mail Answers
Answers were Sorted based on User's Feedback
Answer / abhijeet gavali
You have two different methods for it
1.0 Use Propyl parabean in methanol and pure methanol
2.0 use 0.05% solution of acetone in water and water
| Is This Answer Correct ? | 14 Yes | 3 No |
Mobile phase A is 5.6mg/l of propylparaben in methanol (port
A and B)
Mobile phase B is 100% methanol (port C and D)
Run the test for 20 min by using curve 11 method
sample is Methanol
Acceptance criteria:
A/B/C is between 9.5 % to 10.5
A/B/D is between 9.5 % to 10.5
| Is This Answer Correct ? | 13 Yes | 5 No |
Answer / kuchela rao athota
in waters gradient program
TIME PUMPA% pUMPB% PUMPC% PUMPD% CURVE
0.00 50% 50% 0 0
2.00 0% 0% 50 50 11
6.00 50% 50% 0% 0 11
10.00 45% 45% 10 0 11
12.00 50% 50% 0 0% 11
14.00 45% 45% 0 10 11
16.00 50% 50% 0 0 11
18.00 50% 50% 0 0 11
| Is This Answer Correct ? | 8 Yes | 6 No |
Answer / prerna
Gradient Programme for port A and C :
00 : 100
00 : 100
25 : 75
25 : 75
50 : 50
50 : 50
75 : 25
75 : 25
100 : 00
100 : 00
Above programme also run for port B and D
An increasing stepwise piramid will appear. Integrate that
peak and also split each step of the peak.
Find out its height and area of height
By using the formula ( which I don't remeber ), mean flow
of solvent at each step.
Flow : Limit
25 (24-26)
50 (49-51)
75 (74-76)
100 (100)
If the observed value is within the acceptance limit then
that pump passes the test.
| Is This Answer Correct ? | 10 Yes | 10 No |
What is the column performance and column efficiency?
why we are using in ph calibrations ph4,7,9.2 buffers only?
In Force degradation Study Compound is not degrade in any condition , also in higher stress what can i do ?
We use hicrome 10 cxs column in hplc analysis....pls tell me.. what is the meaning of 10 cxs?
why cone formation during dissolution?
why we multiply 1000 in the determination of kf factor ?
3 Answers Stellence Pharm Science,
What is the column performance and column efficiency?
what is the use of tap density apparatus? why we calculate it? what is the difference between the bulk density and tap density?
1 Answers Alchemist, Alembic, Apex,
What is (Q) point dissolution?
why did uyou choose analytical chemistry?
How to calibrate glassware against std calibrated glassware?
What is the thickness of polystyrene film?
8 Answers Pharmaffiliates Analytics and Synthetics,
Organic Chemistry 
