Why acetonitrile and water are used as extraction solvent when analysis melamine? I thought they are miscible and won't be able to separate...
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If we have 120 minutes run time method for HPLC and you wan to decrease the Run time so what should you do??
in which situation require to change rs specification?
why disodium tartarate is used for autotitrator calibration
Wath is the differenc between two column HPLC wich have the samme support , lenth , pore size, etc.. But different name .for exemple : Spherisorb / lichrosorb
Optical Rotation*0.9477*100*200] Assay Of dextrose= ________________________________ 99.8* 200 why Factor=0.9477
1 Answers Claris Lifesciences, Inven Pharmaceuticals, Levo,
Why using 0.5% acetone in methanol mobile phase for HPLC Gradient performance check.
on saturation solubility study data how we can find out the bcs class of drug?
why 0.005 M sulphuric acid is used to calbrate spectrophotometer and why caffiene is used for calibrating hplc
2 Answers GVK, Torrent Pharma,
In HPLC calibration, caffeine is used as primary standard for wave length calibration due to caffeine is having dual maxima at 273 & 205 nm and one minima at 245 nm. Any body can give reference of these details from any pharmacopeia (with chapter no.) or any other authentic guideline?
what is primary solution and secondary solution
from compund pka value how to select buffer and column?
2 Answers Emcure, Zydus Cadila,
can some other acids like nitric acid or HCl can be used in the calibration of UV.Vis. spectrophotometer????