WHAT IS UNIT OF ABSORBANCE AREA IN HPLC OR UV DETECTER
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Many times I don't got a caffeine peak in calibration of hplc using guard column ❓
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Dear Sir, This is Thirunavukkarasu am working in Ordain Health Care AR&D, Am Having one problem While doing method development my question Metadoxine and Pyridoxine same chemical nature and structural activity i tried lot of methods but Analyte eluted same Retantion time i cant separate both peaks. solubility, pH both are same only only difference melting point metadoxine 210 C Pyridoxine 102 C please do the help needful for me
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U have 4 strengths have different coating meterial which one u chose to do forced degradation stady in validation
Organic Chemistry 
