reasons for negative peaks in chromatography
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Answer / laxman rapolu
where as In UV-absorbance detection : absorbance of solute
less than that of mobile phase. It can be avoided by use of
mobile phase with lower UV absorbance
| Is This Answer Correct ? | 17 Yes | 1 No |
Answer / falguni patel
1. Difference in the composition of the sample and mob phase.
2. Often may sample contains extraneous ingredients such as excipients.
3. Sample may not exactly dissolve in mob phase.
| Is This Answer Correct ? | 0 Yes | 4 No |
Answer / ramakrishna,micro labs
Based on mobile phase negative peaks we observed,for ex: the mobile phase having TEA,Acetic acid or OPA (those all r used for mobile phase pH adjustment) .that type of chemicals will show negative response in HPLC
| Is This Answer Correct ? | 1 Yes | 7 No |
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