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why we are using in ph calibrations ph4,7,9.2 buuffers only?
- 3 Answers
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- Reddy Labs, Unichem, I also Faced
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Answer / guest
1.Calibrations not limited to 4,7,9.2 it's should cover ur
analysis range i.e. minimum to minimum and higher to higher
( 1.68-12.45)
2.Calibrations buffer pH range 0-14.If the drug choose in
acetic nature so the calibrated pH range 1.68 to 4.00
buffer.If the drug choose in alkali nature the calibrated
pH range 9.2 to 12.45. If the drug choose nutral range the
calibrated pH range 6.86 to 7.0 analysis range i.e.
minimum to minimum and higher to higher
( 1.68-12.45)
3.cause acidic ,neutral,basic
| Is This Answer Correct ? | 34 Yes | 1 No |
Answer / amar
pH range is 0-14 where as 7 is the neutral. So, In Acidic range below 7 and Basic range above 7 range we can do the calibration. For Acidic condition 0-7, 4 is the moderate pH. Where as in Basic Condition 7-14, 9.2 is the moderate pH. So, for these conditions we are performing 4,7 and 9.2 buffers for calibration. For pH Calibration we can extend calibration 1.65,4.01,7.00,9.25 and 12.45 for getting more accuracy in pH.
| Is This Answer Correct ? | 15 Yes | 3 No |
Answer / basha
Most of HPLC Analysis Are in range 4-9 pH hence most of the
labs prefer constant buffers . Columns may degrade below
4pH and above pH range hence specific range
| Is This Answer Correct ? | 0 Yes | 7 No |
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