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When i inject amines and acid on HPLC then which peak will
elut first?
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Answer / manjeera
Elution of the compound depends on the type of chromatography whether it is reverse phase or normal phase chromatography.in reverse phase mobile phase will be polar so polar compound elutes first and vice versa for normal phase chromatography.
| Is This Answer Correct ? | 29 Yes | 1 No |
Answer / m.sivalingeswara rao
Elution Order depedands on the polarity of the compound and
with the type of chromatography.Again ,If you choose
Reverse phase/normal phase chromatography, mobile phase
composition ,stationery phase, column oven temperature will
also play key role in deciding the elution order as well as
separation.
| Is This Answer Correct ? | 7 Yes | 0 No |
Answer / dhramveer
Elution of amine and Acid depends upon pH of mobile phase and pKa of molecule. If the mobile phase pH is acidic then Amine will elute first but if the pH is alkaline >7 , then acid may elute first.
| Is This Answer Correct ? | 7 Yes | 1 No |
Answer / ratan
Acid WILL COME FIRST SINCE IT IS POLAR IN NATURE.
| Is This Answer Correct ? | 5 Yes | 6 No |
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