If we are getting split peak in Chromatogram,what we can do?

Categories >> Sciences >> Chemistry >> Analytical Chemistry
Suggest New Category

Question Posted / siri

7 Answers
18748 Views
I also Faced
E-Mail Answers

Answers were Sorted based on User's Feedback

If we are getting split peak in Chromatogram,what we can do?..

Answer / alpesh patel

It means yr column is contaminated & the column needs
proper washing with suitable solvents. You can also
regenarate the column by proper washing with
polar solvent -> semi polar solvent -> non polar solvent ->
semi polar solvent -> polar solvent.

Is This Answer Correct ?

18 Yes

4 No

If we are getting split peak in Chromatogram,what we can do?..

Answer / mr. bujji reddy kanchi

The peak split involves both mobile phase and stationery
phase. The pH of the mobile phase accuracy depends.

Mainly Stationery phase deterioration. Column has to be
regenerated according to the Column certificate
recommondation solvents.

Is This Answer Correct ?

6 Yes

0 No

If we are getting split peak in Chromatogram,what we can do?..

Answer / satyasri

if u observe peak splitting,first u go to change(reduce) injection volume(means volume over load)...dn u go for buffer srength(means conc)...dn u go for pH of the buffer...dn only u go for checking of column performance(means whether u hv to check column was washed properly from previos one)........k

Is This Answer Correct ?

2 Yes

1 No

If we are getting split peak in Chromatogram,what we can do?..

Answer / jayalakshmi

If the sample diluent is not compatable with mobile phase,
it could be the reason for peak splitting. Using mobile
phase as sample diluent would resolve the issue.

Is This Answer Correct ?

1 Yes

0 No

If we are getting split peak in Chromatogram,what we can do?..

Answer / g.balaji

Main cause : That means u r column is contaminated
absorbant ion of impurites in the column.

Solution :
1)Wash calumn:-By polar solvent -> semi polar solvent -> non
polar solvent ->semi polar solvent -> polar solvent.
2)Check column performance
3)Replace column

Is This Answer Correct ?

0 Yes

3 No

If we are getting split peak in Chromatogram,what we can do?..

Answer / umesh patel

BECOZ OF COLUMN OVERLOADING
DECREASE THE INJECTION VOLUME AND CHECK OUT

ALSO
IT HAPPENS THAT IMPURITIES FROM PREVIOUS INJECTIONS ELUTED

RUN BLANK WITH PROPER SOLVENT AND THEN WITH MOBILE PHASE

Is This Answer Correct ?

1 Yes

5 No

If we are getting split peak in Chromatogram,what we can do?..

Answer / n.s.r

In Method development time or already validated method first we conform that already validated method it's ok but in development time we go on peak purity and finalise column problem or if any impurities is present in main peak.Thank you ANY WAY YOU WILL answer that question.

Is This Answer Correct ?

0 Yes

6 No

Post New Answer

More Analytical Chemistry Interview Questions

What will be the effect on HPLC analysis result if three mobile phases given separately through three valves and same proportion mixed together and given by one valve?

1 Answers LOIL,

How to determine the EDTA content by potentiometry titration in Ceftriaxine sodium

0 Answers

what is the extinction coefficient for Indomethacin or at 319nm wave length.

0 Answers GSK GlaxoSmithKline,

what is the difference between melting range & melting point? Which is more significant?

5 Answers

why we cannot analysis below 200nm in u.v?

2 Answers

what is mass spectra ? why it is necessary ?

1 Answers

How to know HPLC column performance?