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When i inject amines and acid on HPLC then which peak will
elut first?
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Answer Posted / m.sivalingeswara rao
Elution Order depedands on the polarity of the compound and
with the type of chromatography.Again ,If you choose
Reverse phase/normal phase chromatography, mobile phase
composition ,stationery phase, column oven temperature will
also play key role in deciding the elution order as well as
separation.
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