Interested to Buy Any Domain ? << Click Here >> for more details...
What is the difference between uplc and hplc
- 4 Answers
- 26478 Views
- AAU Assam Agricultural University, Hetero, I also Faced
- E-Mail Answers
Answers were Sorted based on User's Feedback
Answer / satheesh kumar
HPLC,UPLC both are belongs to liquid chromartography.
HPLC-Longer Run time,Pressure withstands-5000psi,low id not
possible
UPLC-Less Run time,Pressure withstands more than
5000psi,low id possible
| Is This Answer Correct ? | 23 Yes | 3 No |
UPLC is advance varsion of HPLC.
UPLC( ULTRA HIGH PERFORMANCE CHROMATOGRAPHY) is actually initiation of a new era in drug analysis with high pressure, sepration,resulution & most important speed.
uplc is patent by WATERS,whereas SHIMANDZU also on tha way by lunchimg NEXERA.
Overall UPLC is a fast version of HPLC.
| Is This Answer Correct ? | 21 Yes | 4 No |
Answer / k.lavanya
HPLC is high performance liquid chromatography, whereas UPLC is ultra high performance liquid chromatography
In essence, UPLC is just a special variant of HPLC
The pump pressure, which is 40Mpa (400 atmospheres) in HPLC, can go up to 100MPa in UPLC. UPLC also allows for much smaller particle size, normally less than 2um whereas it is normally 5um in HPLC.
| Is This Answer Correct ? | 12 Yes | 1 No |
Answer / khushali senghani
in UPLC productivity is increased and results are obtained mor faster with more resolution whereas in HPLC high pressure is required for solvent flow.
| Is This Answer Correct ? | 0 Yes | 3 No |
what is DIPE limit in residual solvent?
are there in uv lamb that is chractrestic for wavelenghth 205 nm ?????
1 Answers Beni Suef University, Graviti Pharmaceuticals, Sri Krishna Pharmaceuticals,
Hi sir if any product monograph not given known impurities (may be 5 impurities) specifications then how we require to proceed for inhouse formulation?
stability study is going on up to 3years for famotidine-USP but the pharmacopeia is revised and tlc test is replaced with hplc test. At the 4th year frequency the product is failed in hplc test .how can i assain the expiry or retest date and can i stop or continue the programme?
For LOD and LOQ determination which method is accurate? (slopemethod or s/n ratio method)
why we are using buffer solutionas as a mobile phase in hplc.
which chromatographic technique is used for softening hard water?
What are the different zones in stability according to ICH?
what is the concept for adjustment of pH in HPLC buffer solution ? a. Common ion effect b. Counter ion effect and explain me, how we adapt this principles & what is the role of these principles in our method
1 Answers Apex, Pentagon Labs,
Why do we use sodium salicylate for Calibration TLC Cabinet U.V Lamps
Why 1800 ml of dissolution media used In Carbamazepine ER USP Tablets But other drug only 900 ml Media Used ?
For limit test of heavy metals in BP, Method C require that the substance is ignited at a temperature not exceeding 800 °C. Why confines such the temperature?
Organic Chemistry 
